100 ul human peripheral blood were surface stained with 20.00 ul FITC Anti-Human CD34 (FITC-65183, Clone: QBEnd-10) and then treated with red blood cell lysis buffer. Cells were not fixed.
1X10^6 mouse splenocytes were surface co-stained with Anti-Mouse CD3ε and 0.25 ug FITC Anti-Mouse CD90.2 (FITC-65088, Clone:30-H12) or 0.25 ug Control Antibody. Cells were not fixed.
100 ul human peripheral blood were surface stained with 20.00 ul PE Anti-Human CD34 (PE-65183, Clone: QBEnd-10) and 5 ul APC Anti-Human CD45 (APC-65109, Clone: HI30), and then treated with red blood cell lysis buffer. Cells were not fixed.
1X10^6 BALB/c mouse thymocytes were surface stained with 1 ug Anti-Mouse CD90.2 (65088-1-Ig, Clone: 30-H12) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) at dilution 1:1000 (green), or stained with 1 ug Rat IgG2b Isotype Control and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.
1X10^6 human peripheral blood monocytes were surface stained with 5 ul CoraLite®488 Anti-Human CD163 (CL488-65169, Clone:GHI/61) (red), or Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were not fixed .
1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD163 (PE-65169, Clone: GHI/61) (red) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD163 (PE-65169, Clone: GHI/61) or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 TF-1 cells were surface stained with 1.5 ug Anti-Human CD117 (65154-1-Ig, Clone:104D2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 1.5 ug Mouse IgG1 Isotype Control Antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.
