Human red blood cells were surface stained with Anti-Human CD235a (65174-1-Ig, Clone:HIR2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (green), or stained with Mouse IgG2b Isotype Control Antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (black). Cells were not fixed.

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1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD24 (65121-1-Ig, Clone: M1/69) and FITC anti-rat IgG2b Antibody at dilution 1:200 (green), or stained with 0.5 ug isotype control and FITC anti-rat IgG2b Antibody at dilution 1:200 (black). Cells were not fixed.

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1X10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse TER-119 (65149-1-Ig, Clone: TER-119) and FITC Anti-Rat IgG2b Antibody at dilution 1:200 (green), or stained with 0.5 ug Rat IgG2b Isotype Control and FITC Anti-Rat IgG2b Antibody at dilution 1:200 (black). Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.06 ug APC-Rat IgG2b isotype control (left) or 0.06 ug APC Anti-Mouse CD24 (APC-65121, Clone: M1/69) (right). Cells were not fixed.

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1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD45 (FITC-65087, Clone: 30-F11) and 0.50 ug APC Anti-Mouse TER-119 (APC-65149, Clone: TER-119). Cells were not fixed.

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1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD45 (FITC-65087, Clone: 30-F11) and 0.50 ug APC-Rat IgG2b isotype control antibody. Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-conjugated rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD24 (CL488-65121, Clone: M1/69) (right). Cells were not fixed.

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1X10^6 C57BL/6 mouse bone marrow cells were surface stained with APC-Anti-Mouse CD45 (APC-65087, Clone: 30-F11) and 5.00 ul CoraLite®488-conjugated Anti-Mouse TER-119 (CL488-65149, Clone: TER-119). Cells were not fixed.

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1X10^6 C57BL/6 mouse bone marrow cells were surface stained with APC-Anti-Mouse CD45 (APC-65087, Clone: 30-F11) and CoraLite®488-conjugated Rat IgG2b isotype control antibody. Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse CD24 (CL647-65121, Clone: M1/69). Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and CoraLite® Plus 647-conjugated rat IgG2b isotype control antibody. Cells were not fixed.

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