1X10^6 human peripheral blood platelets were surface stained with 10 ul FITC Anti-Human CD9 (FITC-65070, Clone: MM2/57) (green) or FITC-mouse IgG2b isotype control (black). Cells were not fixed.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD9 (65070-1-Ig, Clone: MM2/57) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug mouse IgG2b isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

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1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 (CL488-65195, Clone:5A6) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 (CL488-65195, Clone:5A6), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Human CD81 (CL647-65195, Clone: 5A6) (blue) or isotype control antibody (black). Cells were not fixed.

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1X10^6 human peripheral blood platelets were surface stained with 10 ul PE Anti-Human CD9 (PE-65070, Clone:MM2/57) (red) or PE-mouse IgG2b isotype control (black). Cells were not fixed.

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100 ul human peripheral blood were surface stained with 20.00 ul FITC Anti-Human CD34 (FITC-65183, Clone: QBEnd-10) and then treated with red blood cell lysis buffer. Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD31 (FITC-65058, Clone:390) (red), or 0.5 ug Isotype Control. Cells were not fixed.

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Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using FITC Anti-Mouse CD31 (FITC-65058, Clone: 390) at dilution of 1:100.

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