1X10^6 mouse bone marrow cells were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (FITC-65055, Clone:M1/70) or unstained. Cells were not fixed.
1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16/32 (65057-1-Ig, Clone:93) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed .
1X10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug FITC Anti-Mouse CD80 (B7-1) (FITC-65076, Clone:16-10A1) (red), or 0.5 ug Isotype Control. Cells were not fixed.
1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.125 ug FITC Plus Anti-Mouse CD86 (FITC-65068, Clone: GL1). Cells were not fixed.
1X10^6 LPS treated mouse splenocytes were surface stained with 0.125 ug FITC Plus Anti-Mouse CD86 (FITC-65068, Clone: GL1) (red) or 0.125 ug FITC-rat IgG2a isotype control. Cells were not fixed.
1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul APC Anti-Human PD-L1 (B7-H1) (APC-65081, Clone: 29E.2A3) (blue) or isotype control antibody (black). Cells were not fixed.
1X10^6 mouse splenocytes were surface co-stained with FITC Anti-Mouse MHC Class II (I-A/I-E) and 0.2 ug APC Anti-Mouse CD11c (APC-65130, Clone: N418) or 0.2 ug Isotype Control. Cells were not fixed.
1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 488 Anti-Mouse CD3 and 0.2 ug APC Anti-Mouse CD8a (APC-65069, Clone:53-6.7) or 0.2 ug Isotype Control. Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD31 (FITC-65058, Clone:390) (red), or 0.5 ug Isotype Control. Cells were not fixed.
Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using FITC Anti-Mouse CD31 (FITC-65058, Clone: 390) at dilution of 1:100.
