1X10^6 MC/9 cells were surface stained with 0.25 ug Anti-Mouse FceR1 (65091-1-Ig, Clone: MAR-1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug Armenian Hamster IgG isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.