Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Biolayer interferometry (BLl) kinetic assays of 83594-4-RR
against Human SENP1 were performed. The affinity constant is below 1 pM.
Various lysates were subjected to SDS PAGE followed by western blot with CL594-66009 (Beta Actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.
1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594-conjugated Beta Actin Monoclonal antibody (CL594-66009, Clone:2D4H5)(red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB result of PEX14 antibody (10594-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX14 transfected HEK-293 cells.
Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEX14 antibody (10594-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).
IP result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (10594-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with 83594-4-RR (SENP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83594-4-RR (SENP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83594-4-PBS in a different storage buffer formulation.
Biolayer interferometry (BLl) kinetic assays of 83594-4-RR
against Human SENP1 were performed. The affinity constant is below 1 pM.
Enhancement of RFP signal with RFP-Booster_Atto594. Comparison of signal intensity of a cell line stably expressing a nuclear RFP-fusion protein before and after RFP-Booster treatment. Microscope: InCell1000.
1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug Coralite® Plus 594 IL-1RL1/ST2 Polyclonal Antibody (CL594-30194)(red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-TIPE2 (IP:15940-1-AP, 4ug; Detection:15940-1-AP 1:2000) with mouse spleen tissue lysate 1400 ug.
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIPE2 antibody (15940-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TIPE2 (15940-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
pig tonsil tissue was subjected to SDS PAGE followed by western blot with 11594-1-AP (ACP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.
Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Heavy chain of Rabbit IgG antibody (CL594-66467, Clone: 1C9A5 ) at dilution of 1:500.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15594-1-AP (CDCA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CDCA3 antibody (15594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CSF1R antibody (25949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25949-1-AP (CSF1R Antibody) at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25949-1-AP (CSF1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with CL594-60004 (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GAPDH (CL594-60004, Clone:1E6D9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PEX14 antibody (CL594-10594) at dilution of 1:400, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).
1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human PEX14 (CL594-10594) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with 15949-1-AP (DYNC2LI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 25944-1-AP (PTPN13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25944-1-AP (PTPN13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 10x lens).
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP result of anti-CAPZA2 (IP:15948-1-AP, 4ug; Detection:15948-1-AP 1:800) with mouse heart tissue lysate 4000ug.
Various lysates were subjected to SDS PAGE followed by western blot with CL594-60008 (Beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.
Various lysates were subjected to SDS PAGE followed by western blot with CL594-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:100.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated version of this antibody, CL594-66031 (alpha Tubulin antibody), at dilution of 1:100.
1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Alpha Tubulin (CL594-66031, Clone:1E4C11) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
