Various lysates were subjected to SDS PAGE followed by western blot with 27120-1-AP (NOS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
bEnd.3 cells were subjected to SDS PAGE followed by western blot with 27120-1-AP (eNOS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 27120-1-AP (eNOS antibody) at dilution of 1:450 incubated at room temperature for 1.5 hours.
Non-treated, VEGF (HZ-1038), EGF and Cobalt Chloride treated HUVEC cells were subjected to SDS PAGE followed by western blot with 28939-1-AP (Phospho-NOS3 (Thr495) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 40x lens).
IP result of anti-NOSTRIN (IP:20116-1-AP, 4ug; Detection:20116-1-AP 1:500) with human placenta tissue lysate 1600ug.
Immunofluorescent analysis of HepG2 cells using 20116-1-AP (NOSTRIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27979-1-AP (NOSIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
