Various lysates were subjected to SDS PAGE followed by western blot with CL594-66009 (Beta Actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.
1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594-conjugated Beta Actin Monoclonal antibody (CL594-66009, Clone:2D4H5)(red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-80010, Clone: 4K14 ) at dilution of 1:200.
Various lysates were subjected to SDS PAGE followed by western blot with CL488-66240 (Beta Tubulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66240 (beta Tubulin antibody) at dilution of 1:100.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Beta Tubulin (CL488-66240, Clone:1D4A4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Heavy chain of Rabbit IgG antibody (CL594-66467, Clone: 1C9A5 ) at dilution of 1:500.
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD206 antibody (CL488-60143, Clone: 2A6A10 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD206 antibody (CL488-60143, Clone: 2A6A10 ) at dilution of 1:200.
1X10^6 mouse splenocytes were surface co-stained with CoraLite®594 Anti-Mouse CD3 and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD8a (CL488-65069, Clone: 53-6.7) or 0.5 ug Isotype Control. Cells were not fixed.
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD8a (CL488-65069, Clone: 53-6.7) at dilution of 1:500.
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 GFAP antibody (CL488-60190, Clone: 4B2E10 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 GFAP antibody (CL488-60190, Clone: 4B2E10 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66005 (6*His, His-Tag antibody), at dilution of 1:100.
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66467 (Heavy chain of Rabbit IgG antibody), at dilution of 1:400.
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 VE-cadherin antibody (CL594-66804, Clone: 4F9B10 ) at dilution of 1:100.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (CL488-65055, Clone:M1/70), and 0.25 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2), and 0.25 ug APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (APC-65140, Clone: RB6-8C5). Cells were not fixed .
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD11b (CL488-65055, Clone: M1/70) at dilution of 1:400.
1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD4 (RM4-5) (CL488-65141, Clone:RM4-5) or 0.25 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5) at dilution of 1:500.
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ACE2 antibody (CL488-66699, Clone: 2F12A4 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ACE2 antibody (CL488-66699, Clone: 2F12A4 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD11c/Integrin Alpha X antibody (CL488-60258, Clone: 2F1C10 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD11c/Integrin Alpha X antibody (CL488-60258, Clone: 2F1C10 ) at dilution of 1:200.
Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-66065, Clone: 2A1E2 ) at dilution of 1:400.
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD8a antibody (CL488-66868, Clone: 1G2B10 ) at dilution of 1:200, CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10, red).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®488-conjugated human IgM antibody (CL488-66484, Clone: 2D10B10 ) at dilution of 1:100.
Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 NeuN antibody (CL488-66836, Clone: 3A4C1 ) at dilution of 1:200.
1X10^6 U-87 MG cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NeuN (CL488-66836, Clone:3A4C1) (red), or 0.4 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NeuN (CL488-66836, Clone:3A4C1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).