Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using CoraLite® Plus 488 Phospho-EIF2S1 (Ser51) antibody (CL488-68023, Clone: 1A4A11 ) at dilution of 1:200.
1x10^6 Calyculin A treated PC-3 cells were intracellularly stained with 0.13 ug CoraLite® Plus 488-conjugated Phospho-EIF2S1 (Ser51) Monoclonal antibody (CL488-68023, Clone:1A4A11)(red), or 0.13 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SMN antibody (CL488-11708) at dilution of 1:200, CL594-Phalloidin (red).
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SMN Polyclonal antibody (CL488-11708)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 TMEM173/STING antibody (CL647-80165, Clone: 7K7 ) at dilution of 1:200.
Various lysates were subjected to SDS PAGE followed by western blot with CL594-66009 (Beta Actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5 ) at dilution of 1:200.
1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594-conjugated Beta Actin Monoclonal antibody (CL594-66009, Clone:2D4H5)(red), or 0.8 ug CoraLite®594 Mouse IgG2b Isotype Control (MPC-11) (CL594-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with CL488-66240 (Beta Tubulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66240 (beta Tubulin antibody) at dilution of 1:100.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Beta Tubulin (CL488-66240, Clone:1D4A4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 ANGPTL4 antibody (CL488-67577, Clone: 1D2H6 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Heavy chain of Rabbit IgG antibody (CL594-66467, Clone: 1C9A5 ) at dilution of 1:500.
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD206 antibody (CL488-60143, Clone: 2A6A10 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD206 antibody (CL488-60143, Clone: 2A6A10 ) at dilution of 1:200.
Various lysates were subjected to SDS PAGE followed by western blot with CL488-66009 (Beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 Beta Actin antibody (CL488-66009, Clone: 2D4H5 ) at dilution of 1:200.
(blue)1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Beta Actin (CL488-66009, Clone:2D4H5)(red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PEX14 antibody (CL594-10594) at dilution of 1:400, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).
1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human PEX14 (CL594-10594) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.
Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Alpha Tubulin (CL488-66031, Clone:1E4C11) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 mouse splenocytes were surface co-stained with CoraLite®594 Anti-Mouse CD3 and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD8a (CL488-65069, Clone: 53-6.7) or 0.5 ug Isotype Control. Cells were not fixed.
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD8a (CL488-65069, Clone: 53-6.7) at dilution of 1:500.
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 GFAP antibody (CL488-60190, Clone: 4B2E10 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 GFAP antibody (CL488-60190, Clone: 4B2E10 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200, CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOM20 (CL488-66777, Clone:1D6F5) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TOM20 antibody (CL488-66777, Clone: 1D6F5 ) at dilution of 1:200, CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 TOM20 antibody (CL488-11802) at dilution of 1:200, CL594-Phalloidin (red).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOM20 (CL488-11802) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11 ) at dilution of 1:100, CoraLite®488 Cardiac Troponin I antibody (CL488-66376, Clone: 1D5D6, green), CoraLite®594 N-cadherin antibody (CL594-22018, red). DAPI (blue).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11 ) at dilution of 1:200.
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human smooth muscle actin specific (CL647-67735, Clone:1E9A11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with CL594-60008 (Beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Beta Actin antibody (CL594-60008, Clone: 7D2C10 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66005 (6*His, His-Tag antibody), at dilution of 1:100.
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL488-66200 (GREEN, Acetyl-Tubulin (Lys40) antibody) at dilution of 1:50 and CL594-17711 (RED, ARL13B antibody) at dilution of 1:50.
Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD-31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD-31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB.
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 Acetyl-Tubulin (Lys40) antibody (CL488-66200, Clone: 7E5H8 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).
