Various lysates were subjected to SDS PAGE followed by western blot with CL750-82929-2 (MAPKAPK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Non-transfected and transfected HEK-293T lysates were subjected to SDS PAGE followed by western blot with HRP-81290 (HA tagged antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 647 IBA1 antibody (CL647-81728, Clone: 4D5 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 647 IBA1 antibody (CL647-81728, Clone: 4D5 ) at dilution of 1:200.
1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human IBA1 (CL647-81728, Clone:4D5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 TMEM173/STING antibody (CL647-80165, Clone: 7K7 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.
Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human smooth muscle actin specific (CL594-80008, Clone:5H7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD11b and 5 ul APC Anti-Human CD14 Rabbit Recombinant Antibody (APC-98040, Clone: 230332D7) or APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD14 Rabbit Recombinant Antibody (APC-98040, Clone:230332D7) (red) or APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD19 and 5 ul APC CD3 Recombinant Antibody (APC-65569, Clone: OKT3) or APC Mouse IgG2a Isotype Control (APC-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD19 and 5 ul APC Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (APC-65570, Clone: UCHT1) (right), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (APC-65570, Clone: UCHT1) (red), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (blue). Cells were not fixed.
1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-98042, Clone: 240427E12) or APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD4 Rabbit Recombinant Antibody (APC-98042, Clone:240427E12) (red) or APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.
1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CD45 Rabbit Recombinant Antibody (APC-98117, Clone: 241670E10) or APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human CD45 Rabbit Recombinant Antibody (APC-98117, Clone:241670E10) (red) or APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD45RA and 5 ul APC Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody (APC-65613, Clone: UCHL1) (right), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45RO (UCHL1) Mouse IgG2a Recombinant Antibody (APC-65613, Clone: UCHL1) (red), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (blue).Cells were not fixed.
1x10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 5 ul APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4) or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4) (red), or APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.
1x10^6 untreated or PHA treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul APC Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (APC-65586, Clone: EH12.2H7). Cells were not fixed.
1x10^6 PHA treated human PBMCs were surface stained with 5 ul APC Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (APC-65586, Clone: EH12.2H7) (red) or APC Mouse IgG2a Isotype Control (APC-65208, Clone: C1.18.4) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul APC Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (APC-65586, Clone: EH12.2H7) (black, dashed). Cells were not fixed.
1x10^6 human peripheral blood leukocytes were unstained (left, blue in histogram) or surface stained with 0.1 ug APC Rabbit IgG Isotype Control Recombinant Antibody (APC-98136, Clone: 240953C9) (mid, red in histogram), or 0.1 ug APC Anti-Human CD45 (HI30) (APC-65109, Clone: HI30) (right, green in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 5 ul APC-Cyanine7 CD11a Recombinant Antibody (APC-Cyanine7-65563, Clone: TS2/4) or unstained. Cells were not fixed.
1x10^6 human peripheral blood leukocytes were unstained or surface stained with 5 ul APC-Cyanine7 Anti-Human CD45 Rabbit Recombinant Antibody (AY7-98117, Clone: 241670E10). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human peripheral blood leukocytes were unstained (blue) or surface stained with 5 ul APC-Cyanine7 Anti-Human CD45 Rabbit Recombinant Antibody (AY7-98117, Clone: 241670E10) (red). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 human PBMCs were surface stained with 5 ul APC-Cyanine7 CD73 Recombinant Antibody (APC-Cyanine7-65564, Clone: AD2) or unstained. Cells were then stained with 5 ul CoraLite® Plus 488 Anti-Human CD3 (OKT3) (CL488-65133, Clone: OKT3). Cells were not fixed.
1x10^6 human PBMCs were surface stained with 5 ul APC-Cyanine7 CD8 Recombinant Antibody (AY7-65559, Clone: UCHT4) or unstained. Cells were then stained with 5 ul PE Anti-Human CD3 (UCHT1) (PE-65151, Clone: UCHT1). Cells were not fixed.
1x10^6 human PBMCs were surface stained with 5 ul APC-Cyanine7 HLA-DR Recombinant Antibody (AY7-65560, Clone: L243) or unstained. Cells were then stained with 5 ul CoraLite® Plus 488 Anti-Human CD19 (HIB19) (CL488-65110, Clone: HIB19). Cells were not fixed.
1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD2 Rabbit Recombinant Antibody (Biotin-98134, Clone: 241222E2) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD2 Rabbit Recombinant Antibody (Biotin-98134, Clone: 241222E2) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
