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Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble Biotin Kit (KFA111) and streptavidin-PE dye. The cells are co-stained with anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with CoraLite® Plus 488 (CL488-65197). Note: Cells treated with Endogenous Biotin-Blocking Kit from Thermofisher.
Live A431 cells were immunostained with anti-EGFR (cetuximab biosimilar) labeled with FlexAble Biotin Antibody Labeling Kit for Human IgG (KFA111) and Streptavidin-ATTO594 (red). Nuclei are in cyan. Epifluorescence images were acquired with a 20x objective and post-processed.
Live SKBR-3 cells immunostained with anti-HER2 (trastuzumab biosimilar) labeled with FlexAble Biotin Antibody Labeling Kit for Human IgG (KFA111) and Streptavidin-ATTO594 (red). Nuclei are in blue. Epifluorescence images were acquired with a 20x objective and post-processed.
Immunofluorescent analysis of (4% PFA) fixed human lung cancer using FlexAble Biotin Antibody Labeling Kits for Human IgG and Streptavidin-594(red) to label endogenous human IgG in the tissue.
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Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-CD147 antibody labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-ATTO594 (yellow). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Flow Cyotometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-APC. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246). Cells are not fixed, Monocytes are gated.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-PE. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246). Cells are not fixed, Monocytes are gated.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-PE. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246). Cells are not fixed, Monocytes are gated.
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and used at a dilution of 1:400 (under 10x lens & 40xlens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and used at a dilution of 1:400 (under 10x lens & 40xlens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).
Flow Cyotometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-APC. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246). Cells are not fixed, Monocytes are gated.
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Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 antibody (67169-1-lg) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-ATTO594 (yellow). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-GFAP antibody (60190-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-650 (magenta). Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-GFAP antibody (60190-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-488 (green), and anti-SOX10 antibody (66786-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-594 (red). Cell nuclei are stained with DAPI (blue).
Flow cytometry with PBMC. 1X10^6 human PBMC were stained with either mouse IgG2a isotype control or anti-human CD3 antibody (65133-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and PE-Streptavidin. Cells were co-stained with anti-human CD4 antibody (CL488-65143). Cells are not fixed, lymphocytes are gated.
Flow cytometry with PBMC. 1X10^6 human PBMC were stained with either mouse IgG2a isotype control or anti-human CD3 antibody (65133-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and PE-Streptavidin. Cells were co-stained with anti-human CD4 antibody (CL488-65143). Cells are not fixed, lymphocytes are gated.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with either mouse IgG2a isotype control or anti-human CD8 antibody (65135-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with either mouse IgG2a isotype control or anti-human CD8 antibody (65135-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using anti-CK7 antibody (66483-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and used at a dilution of 1:800 (under 10x lens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using anti-CK7 antibody (66483-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and used at a dilution of 1:800 (under 40x lens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).
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Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin β antibody labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-ATTO594 (yellow). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of human colon: FFPE human colon sections were stained with anti-KRT20 antibody (60183-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-650 (magenta). Cell nuclei are in blue.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD4 antibody (65134-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD4 antibody (65134-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD20 antibody (65085-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD45 antibody (CL488-65109). Cells are not fixed, lymphocytes are gated.
Flow cytometry of PBMC. 1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD20 antibody (65085-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD45 antibody (CL488-65109). Cells are not fixed, lymphocytes are gated.
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Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GM130 (11308-1-AP) labeled with FlexAble Biotin Kit for Rabbit IgG (KFA007) and Streptavidin-ATTO594 (yellow). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 antibody (12987-1-AP) followed by polyclonal goat anti-rabbit AF488 secondary antibody (green), anti-GM130 antibody (11308-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit (KFA007) and Streptavidin-ATTO594 (yellow), and anti-Tom20 antibody (11802-1-AP) separately labeled with FlexAble Biotin Antibody Labeling Kit (KFA007) and Streptavidin-AQ650 (red). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Immunofluorescence of mouse testis: FFPE mouse testis sections were stained with anti-TNP1 antibody (17178-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and Streptavidin-488 (green), and anti-BOLL antibody (13720-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and Streptavidin-594 (red). Cell nuclei are stained with DAPI (blue).
Flow cytometry of PBMC. 1X10^6 HEK293 cells were fixed with FoxP3/Transcription Factor Staining Buffer Kit (PF00011), then stained with anti-human Mitofilin antibody (10179-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and Streptavidin-APC. Single cells are gated.
Flow cytometry of PBMC. 1X10^6 HEK293 cells were fixed with FoxP3/Transcription Factor Staining Buffer Kit (PF00011), then stained with anti-human Mitofilin antibody (10179-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and Streptavidin-PE. Single cells are gated.
Immunohistochemical analysis of paraffin-embedded Rabbit colon tissue using anti-Cytokeratin 20 antibody (17329-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and used at a dilution of 1:1000 (under 10x lens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded Rabbit colon tissue using anti-Cytokeratin 20 antibody (17329-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and used at a dilution of 1:1000 (under 40x lens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).
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Immunofluorescence of A431 cells: PFA-fixed cells were stained with rat anti-Tubulin alpha antibody, labeled with FlexAble Biotin Kit (KFA128) and Streptavidin-647 (magenta). Cell nuclei were stained with DAPI (blue). This epifluorescence image was acquired at Zeiss Demo Center, Munich, with a 40x objective and post-processed.