G66360-3-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G66360-3-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

HeLa cells were immunostained with mouse IgG2b anti-Tubulin antibody (another vendor), labeled with FlexAble 2.0 CoraLite® Plus 405 Kit (KFA0566, cyan). Cell nuclei are in red. Epifluorescence images were acquired with a 20x objective and post-processed. 

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin β antibody labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-ATTO594​ (yellow). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of human colon: FFPE human colon sections were stained with anti-KRT20 antibody (60183-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-650 (magenta). Cell nuclei are in blue.

Flow cytometry of PBMC.  1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD4 antibody (65134-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.

Flow cytometry of PBMC.  1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD4 antibody (65134-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.

Flow cytometry of PBMC.  1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD20 antibody (65085-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD45 antibody (CL488-65109). Cells are not fixed, lymphocytes are gated.

Flow cytometry of PBMC.  1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD20 antibody (65085-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD45 antibody (CL488-65109). Cells are not fixed, lymphocytes are gated.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA066, cyan) and anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA064, red). Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA066).  Cells are not fixed, lymphocytes are gated. 

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green). Nuclei were stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), mouse IgG2b anti-PAF49 primary and anti-mouse IgG secondary antibody Alexa Fluor® 568 (red) and CoraLite® 647-conjugated HDAC2 antibody (CL647-67615, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), mouse IgG2b anti-Lamin primary and anti-mouse IgG secondary antibody Alexa Fluor® 568 (red) and anti-Tom20 (66777-1-Ig) labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, magenta). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, yellow). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA061).​

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA061).​

WB of HeLa and HEK-293 cell lysates: HeLa and HEK-293 Cell lysates were detected with anti-Beta Actin (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit (KFA061, blue); anti-Alpha Tubulin (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA062, red);  and anti-ANP32B (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA064, green).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, orange). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, yellow). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with CoraLite® 488-conjugated Tom20 antibody (CL488-66777, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti- PAF49 antibody labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Actin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-Lamin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062).​

WB of HeLa and HEK-293 cell lysates: HeLa and HEK-293 Cell lysates were detected with anti-Beta Actin (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit (KFA061, blue); anti-Alpha Tubulin (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA062, red); and anti-ANP32B (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA064, green).

Immunofluorescence of A431 cells: PFA-fixed cells were stained with anti-Tubulin (66031-1-Ig) labeled with FlexAble CoraLite® Plus 594 Kit (KFA069, orange) and DAPI (blue). ​ Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 594 Kit (KFA069) and anti-CD3 (CL750-65151). Cells are not fixed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tom20 (66777-1-Ig) labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, magenta). Nuclei were stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HDAC2 (676165-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), mouse IgG2b anti-Lamin primary and anti-mouse IgG secondary antibody Alexa Fluor® 568 (red) and anti-Tom20 (66777-1-Ig) labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, magenta). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, yellow). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with CoraLite® 488-conjugated Tom20 antibody (CL488-66777, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti- PAF49 antibody labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Actin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-Lamin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA063).​

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA063).​

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA066, cyan) and anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA064, red). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tom20 (66777-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite® Plus 750 Kit (KFA064, grey). Cell nuclei were stained with DAPI (blue).  Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA064).  Cells are not fixed, lymphocytes are gated.

Western Blot of HeLa and HEK-293 cell lysates were detected with anti-Beta Actin antibody (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 (KFA061) and anti-ANP32B antibody (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064).

Western Blot of HeLa and HEK-293 cell lysates were detected with anti-ANP32B antibody (66160-1-Ig) labeled with FlexAble CoraLite Plus 488 (KFA061) and anti-Beta Actin antibody (66009-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064).

Western Blot of HeLa and Jurkat cell lysates were detected with anti-Alpha Tubulin antibody (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 (KFA062) and anti-FDPS antibody (67972-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064).

Western Blot of HeLa and Jurkat cell lysates were detected with anti-Alpha Tubulin antibody (66031-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064) and anti-FDPS antibody (67972-1-Ig) labeled with FlexAble CoraLite Plus 555 (KFA062).

Western Blot of HeLa and HEK-293 cell lysates were detected with anti-Beta Actin antibody (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 (KFA061), anti-Alpha Tubulin antibody (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 (KFA062), and anti-ANP32B antibody (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble FITC Plus Kit (KFA068).  Cells are not fixed, lymphocytes are gated.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA066).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble FITC Plus Kit (KFA068).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA064).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-c-SRC antibody (60315-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2b (KFA065).

Various cell lysates were subjected to SDS PAGE followed by western blot with anti-ANP32B antibody (66160-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2b (KFA065).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM20 antibody (66777-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2b (KFA065) and Tyramide-594 (red). Cell nuclei are in blue. 

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, orange), anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561, green) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA503, magenta), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA522, orange), anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561, green) and DAPI (blue).

Immunofluorescence of A431 cells: PFA-fixed cells were stained with mouse IgG2b anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561, green), mouse IgG2b anti-Lamin antibody labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562, yellow), mouse IgG2b anti-HDAC2 antibody (67165-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA563, magenta) and mouse IgG2b anti-Tom20 antibody (66777-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA564, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with mouse IgG2b anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561, green). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD20 (65085-1-Ig, Clone:2H7) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561), and Anti-Human CD45 (65109-1-Ig, Clone:HI30) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD4 (65134-1-Ig, Clone:OKT4) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561), and Anti-Human CD3 (65151-1-Ig, Clone:UCHT1) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta),anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562, orange) and DAPI (blue).

Immunofluorescence of Mouse colon: FFPE Mouse colon sections were stained with anti-FUS (11570-1-AP) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green), anti-α-SMA (67735-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA523, magenta),anti-E-cadherin (60335-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562, orange) and DAPI (blue).

Immunofluorescence of A431 cells: PFA-fixed cells were stained with mouse IgG2b anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561, green), mouse IgG2b anti-Lamin antibody labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562, yellow), mouse IgG2b anti-HDAC2 antibody (67165-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA563, magenta) and mouse IgG2b anti-Tom20 antibody (66777-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA564, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with mouse IgG2b anti-Lamin antibody labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562, red). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD20 (65085-1-Ig, Clone:2H7) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562), and Anti-Human CD45 (65109-1-Ig, Clone:HI30) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD4 (65134-1-Ig, Clone:OKT4) labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562), and Anti-Human CD3 (65151-1-Ig, Clone:UCHT1) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA524). Cells were not fixed. Lymphocytes were gated.

HeLa cells were immunostained with mouse IgG2b anti-TOM20 antibody (66777-1-Ig), labeled with FlexAble 2.0 CoraLite® Plus 594 Kit (KFA569, red). Cell nuclei are in cyan. Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with mouse IgG2b anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561, green), mouse IgG2b anti-Lamin antibody labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562, yellow), mouse IgG2b anti-HDAC2 antibody (67165-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA563, magenta) and mouse IgG2b anti-Tom20 antibody (66777-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA564, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with mouse IgG2b anti-HDAC2 antibody (67165-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA563, magenta). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD20 (65085-1-Ig, Clone:2H7) labeled with FlexAble CoraLite® Plus 647 Kit (KFA563), and Anti-Human CD45 (65109-1-Ig, MsIgG1 Clone:HI30) labeled with FlexAble CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD4 (65134-1-Ig, Clone:OKT4) labeled with FlexAble CoraLite® Plus 647 Kit (KFA563), and Anti-Human CD3 (65151-1-Ig, MsIgG1 Clone:UCHT1) labeled with FlexAble CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with mouse IgG2b anti-PAF49 antibody labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA561, green), mouse IgG2b anti-Lamin antibody labeled with FlexAble 2.0 CoraLite® Plus 555 Kit (KFA562, yellow), mouse IgG2b anti-HDAC2 antibody (67165-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 647 Kit (KFA563, magenta) and mouse IgG2b anti-Tom20 antibody (66777-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA564, cyan). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431 cells: PFA-fixed cells were stained with mouse IgG2b anti-Tom20 antibody (66777-1-Ig) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA564, cyan). Cell nuclei were stained with DAPI (blue). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD20 (65085-1-Ig, Clone:2H7) labeled with FlexAble 2.0 CoraLite® Plus 750 Kit (KFA564), and Anti-Human CD45 (65109-1-Ig, Clone:HI30) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with Anti-Human CD4 (65134-1-Ig, Clone:OKT4) labeled with FlexAble 2.0  CoraLite® Plus 750 Kit (KFA564), and Anti-Human CD3 (65151-1-Ig, Clone:UCHT1) labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA521). Cells were not fixed. Lymphocytes were gated.