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YTHDF1 Polyclonal antibodies
(Parent antibody plus 2 derived formats/conjugates)
- KO/KD Validated
- 246 Publications
- Reviews (8)
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Host/IsoType
Rabbit/IgG
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Reactivity
human, mouse, rat, chicken, monkey, pig, zebrafish
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Applications
CoIP, IF/ICC, IF-P, IHC, IP, RIP, WB
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Conjugation
CoraLite® Plus 488, CoraLite®594, Unconjugated
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Unconjugated parent antibody
Cat No. 17479-1-APOther conjugate/format antibodies
Cat No. CL594-17479, CL488-17479
YTHDF1 Polyclonal antibodies (3 total products)-_- / -_-
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YTHDF1 Monoclonal antibodies (3A2H12)
(Parent antibody plus 1 derived formats/conjugates)
- KO/KD Validated
- 13 Publications
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Host/IsoType
Mouse/IgG1
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Reactivity
human, mouse, rat, pig
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Applications
CoIP, ELISA, IF/ICC, IHC, IP, RIP, WB
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Conjugation
Unconjugated
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CloneNo.
3A2H12
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Unconjugated parent antibody
Cat No. 66745-1-IgOther conjugate/format antibodies
Cat No. 66745-1-PBS
YTHDF1 Monoclonal antibodies (3A2H12) (2 total products)-_- / -_-
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YTHDF1 Recombinant antibodies (241445B10)
(Parent antibody plus 1 derived formats/conjugates)
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Host/IsoType
Rabbit/IgG
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Reactivity
human, mouse, rat
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Applications
Cytometric bead array, ELISA, FC (Intra), IF-P, WB
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Conjugation
Unconjugated
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CloneNo.
241445B10
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Unconjugated parent antibody
Cat No. 80876-2-RROther conjugate/format antibodies
Cat No. 80876-2-PBS
YTHDF1 Recombinant antibodies (241445B10) (2 total products)-_- / -_-
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YTHDF1 Matched Antibody PairsNew
Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.Pair Cat No. Capture antibody Detection antibody Target Species reactivity Validated Applications Linear Range MP01146-4 80876-4-PBS 80876-7-PBS YTHDF1 Human Sandwich ELISA sELISA: 78.1-5000 pg/mL MP01146-1 80876-4-PBS 80876-3-PBS YTHDF1 Human Cytometric bead array CBA: 0.469-60 ng/mL MP01146-2 80876-4-PBS 80876-5-PBS YTHDF1 Human Cytometric bead array CBA: 0.938-60 ng/mL MP01146-3 80876-4-PBS 80876-2-PBS YTHDF1 Human Cytometric bead array CBA: 0.469-60 ng/mL -
× The search results gallery shows a selection of our total image library
More validation images available on the product page
HepG2 cells were subjected to SDS PAGE followed by western blot with 26787-1-AP (YTHDF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP result of anti-YTHDF1 (IP:26787-1-AP, 4ug; Detection:26787-1-AP 1:300) with HepG2 cells lysate 3200ug.
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× The search results gallery shows a selection of our total image library
More validation images available on the product page
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using KHC0157 (YTHDF1 IHC Kit).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using KHC0157 (YTHDF1 IHC Kit).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0157 (YTHDF1 IHC Kit).
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× The search results gallery shows a selection of our total image library
More validation images available on the product page
Various lysates were subjected to SDS PAGE followed by western blot with 80323-1-RR (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 80790-1-RR (METTL14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
4T1 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67811-1-Ig (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 81340-1-RR (YTHDF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF1(m6A reader) antibody 17479-1-AP (1:2000). (Lysate: 3.6mg per IP; IP: 15µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP3 (m6A reader) antibody 66526-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
IP result of anti-WTAP (IP:60188-1-Ig, 4ug; Detection:10200-1-AP 1:8000) with HeLa cells lysate 2000 ug.
IP result of anti-YTHDF1 (IP:66745-1-Ig, 4ug; Detection:66745-1-Ig 1:2000) with Jurkat cells lysate 2000 ug.
IP result of anti-YTHDF2 (IP:81340-1-RR, 4ug; Detection:81340-1-RR 1:1000) with HeLa cells lysate 1880 ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67811-1-Ig (ALKBH5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
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× The search results gallery shows a selection of our total image library
More validation images available on the product page
Various lysates were subjected to SDS PAGE followed by western blot with 80323-1-RR (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 81471-1-RR (FTO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66580-1-Ig (NSUN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 21207-1-AP (TET2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67673-1-Ig (RNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 16727-1-AP (TRMT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Total RNA was isolated from HEK-293 cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with m6A antibody 68055-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using unrelated antibody with the same isotype (UCP2 antibody 66700-1-Ig) at the same dose.
Total DNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with m5C antibody 68301-1-Ig at 1:5000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2b isotype control Monoclonal antibody 66360-3-Ig at the same dose.
Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with m7G antibody 68302-1-Ig at 1:5000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2b isotype control Monoclonal antibody 66360-3-Ig at the same dose.
Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose.
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with YTHDF1(m6A reader) antibody 17479-1-AP (1:2000). (Lysate: 3.6mg per IP; IP: 15µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
HEK-293 cells were lysised and immunoprecipitated with Protein A-m6A antibody and Protein A-mouse IgG3 control antibody respectively in the presence of RNAase inhibotor cocktail. The immunoprecipitated complex was washed diggested by RNAse A followed by western blot with IGF2BP3 (m6A reader) antibody 66526-1-Ig (1:2000). (Lysate: 4.0 mg per IP; IP: 30µg antibody and 50µL beads, 4 hours at 4℃; Diggestion: 50µg/mL * 80µL RNAse A for 1 hour at 37℃; Loading: 20% of elution; Input: 10µg.)
IP result of anti-YTHDF1 (IP:66745-1-Ig, 4ug; Detection:66745-1-Ig 1:2000) with Jurkat cells lysate 2000 ug.
IP result of anti-NSUN2 (IP:66580-1-Ig, 4ug; Detection:66580-1-Ig 1:4000) with HepG2 cells lysate 1920 ug.
IP result of anti-TET2 (IP:21207-1-AP, 3ug; Detection:21207-1-AP 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68055-1-Ig (m6A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68301-1-Ig (m5C antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68301-1-Ig (m5C antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68302-1-Ig (m7G antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68302-1-Ig (m7G antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80323-1-RR (METTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81471-1-RR (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21207-1-AP (TET2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16690-1-AP (ALY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 16727-1-AP (TRMT6 Antibody) at dilution of 1:100 (under 10x lens).
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YTHDF1 Polyclonal antibodies (3 total products)
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YTHDF1 Monoclonal antibodies (3A2H12) (2 total products)
-_- / -_-
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YTHDF1 Recombinant antibodies (241445B10) (2 total products)
-_- / -_-
-
YTHDF1 Matched Antibody PairsNew
Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.Pair Cat No. Capture antibody Detection antibody Target Species reactivity Validated Applications Linear Range MP01146-4 80876-4-PBS 80876-7-PBS YTHDF1 Human Sandwich ELISA sELISA: 78.1-5000 pg/mL MP01146-1 80876-4-PBS 80876-3-PBS YTHDF1 Human Cytometric bead array CBA: 0.469-60 ng/mL MP01146-2 80876-4-PBS 80876-5-PBS YTHDF1 Human Cytometric bead array CBA: 0.938-60 ng/mL MP01146-3 80876-4-PBS 80876-2-PBS YTHDF1 Human Cytometric bead array CBA: 0.469-60 ng/mL -
YTHDF1 E.Coli expressed Tagged-proteins (4 total products)-_- / -_-