Results for "Spot Trap Nanobody"

Spot-Traps

19 Publications

Reviews (1)

Applications

ChIP, CoIP, IP, RIP

Conjugation

Agarose, Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads), Magnetic agarose, Magnetic Particles M-270
-_- / -_-

Cat No. etak, etdk, etmak, eta, etma, etd, etak-iST
Spot-Traps (7 total products)
-_- / -_-
Spot-Labels

4 Publications

Reviews (1)

Applications

IF/ICC

Conjugation

Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647, ATTO 488, ATTO 594
-_- / -_-

Cat No. ebAF568, ebAF488, eba594, eba488, ebAF647
Spot-Labels (5 total products)
-_- / -_-
Spot VHH, recombinant binding protein

6 Publications

Enlarge Image

Application

Conjugation

Conjugate

Unconjugated

Specificity

Spot-Tag^®

Sizes
-_- / -_-

Cat No. etb
Spot VHH, recombinant binding protein
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Western blot with Spot VHH.
pSpot2 vector

2 Publications

View More Images (2)

×
T7 promoter: 1-19 Lac operon: 19-46 RBS: 64-80 MCS: 90-140 Spot-Tag®: 141-176 Stop codon (TAA): 177-179 rrnB terminator: 361-518 Kanamycin resistance gene: 798-1613 pRB322 replication origin: 1781-2400

Vector Type

bacterial expression vector

Promoter

T7

Reporter

Spot-Tag® C-terminal

Selection

kanamycin

Sizes
-_- / -_-

Cat No. ev-2
pSpot2 vector
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

T7 promoter: 1-19 Lac operon: 19-46 RBS: 64-80 MCS: 90-140 Spot-Tag®: 141-176 Stop codon (TAA): 177-179 rrnB terminator: 361-518 Kanamycin resistance gene: 798-1613 pRB322 replication origin: 1781-2400

Multiple cloning site (MCS)
Spot-Cap®

2 Publications

$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.$
View More Images (6)

×
Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.
$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.$

Application

Protein purification

Conjugate

Agarose

Specificity

Spot-Tag^®

Sizes
-_- / -_-

Cat No. eca
$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.$

Spot-Cap®
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.$

Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.

Workflow of Spot-Cap purification.

Spot-Cap has a higher selectivity than anti-DYKDDDDK resin. While Spot-Cap is optimized for minimal contamination of host cell proteins, the anti-DYKDDDDK resin binds significant amounts of mammalian proteins. Incubation of control HEK293T cell lysate expressing no tagged protein with Spot-Cap or anti-DYKDDDDK resin. Elution with 100 μM Spot-peptide or 0.1 g/L DYKDDDDK-peptide. Similar results were obtained for yeast, bacteria, and insect cell lysates (data not shown).

$The elution efficiency of Spot-Cap is constant even after 4 regeneration cycles. Elution of Spot-tagged PCNA with Spot-peptide. R0: Reference (elution fraction of Spot-PCNA without regeneration); R1-R4: Elution fraction of Spot-PCNA after 1, 2, 3, and 4 regeneration cycles with 100 mM glycine pH 2.0.$

The elution efficiency of Spot-Cap is constant even after 4 regeneration cycles. Elution of Spot-tagged PCNA with Spot-peptide. R0: Reference (elution fraction of Spot-PCNA without regeneration); R1-R4: Elution fraction of Spot-PCNA after 1, 2, 3, and 4 regeneration cycles with 100 mM glycine pH 2.0.

$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in batch format. Elution: 6 fractions à 2 BVs Spot-peptide (100 μM) in PBS.$

Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in batch format. Elution: 6 fractions à 2 BVs Spot-peptide (100 μM) in PBS.

Application of Spot-tag related products.
pSpot5 vector

1 Publications

View More Images (2)

×
TPI1 promoter: 7239-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 47-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 CEN replication origin: 3398-3916 Ampicillin resistance gene: 4048-4908 pRB322 replication origin: 5063-5682

Vector Type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® N-terminal

Selection

leucin (S. cerevisiae) Ampicillin (E. coli)6

Sizes
-_- / -_-

Cat No. ev-5
pSpot5 vector
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

TPI1 promoter: 7239-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 47-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 CEN replication origin: 3398-3916 Ampicillin resistance gene: 4048-4908 pRB322 replication origin: 5063-5682

Multiple cloning site (MCS)
Spot-peptide

1 Publications

View More Images (7)

Application

Elution

Conjugate

Unconjugated

Sizes
-_- / -_-

Cat No. ep
Spot-peptide
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Model fig of Spot peptide.

$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.$

Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.

Workflow of Spot-Cap purification.

Spot-Cap has a higher selectivity than anti-DYKDDDDK resin. While Spot-Cap is optimized for minimal contamination of host cell proteins, the anti-DYKDDDDK resin binds significant amounts of mammalian proteins. Incubation of control HEK293T cell lysate expressing no tagged protein with Spot-Cap or anti-DYKDDDDK resin. Elution with 100 μM Spot-peptide or 0.1 g/L DYKDDDDK-peptide. Similar results were obtained for yeast, bacteria, and insect cell lysates (data not shown).

$The elution efficiency of Spot-Cap is constant even after 4 regeneration cycles. Elution of Spot-tagged PCNA with Spot-peptide. R0: Reference (elution fraction of Spot-PCNA without regeneration); R1-R4: Elution fraction of Spot-PCNA after 1, 2, 3, and 4 regeneration cycles with 100 mM glycine pH 2.0.$

The elution efficiency of Spot-Cap is constant even after 4 regeneration cycles. Elution of Spot-tagged PCNA with Spot-peptide. R0: Reference (elution fraction of Spot-PCNA without regeneration); R1-R4: Elution fraction of Spot-PCNA after 1, 2, 3, and 4 regeneration cycles with 100 mM glycine pH 2.0.

$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in batch format. Elution: 6 fractions à 2 BVs Spot-peptide (100 μM) in PBS.$

Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in batch format. Elution: 6 fractions à 2 BVs Spot-peptide (100 μM) in PBS.

Application of Spot-tag related products.
pSpot2 GFPSpot-Tag vector

Enlarge Image

×
T7 promoter: 1-19 Lac operon: 19-46 RBS: 64-80 EGFP-Spot-Tag®: 88-843 Spot-Tag®: 805-840 rrnB terminator: 1025-1182 Kanamycin resistance gene: 1462-2277 pMB1 replication origin: 2445-3064

Vector Type

Bacterial expression vector

Promoter

T7

Reporter

Spot-Tag® C-terminal

Selection

Kanamycin

Sizes
-_- / -_-

Cat No. ev-32
pSpot2 GFPSpot-Tag vector
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

T7 promoter: 1-19 Lac operon: 19-46 RBS: 64-80 EGFP-Spot-Tag®: 88-843 Spot-Tag®: 805-840 rrnB terminator: 1025-1182 Kanamycin resistance gene: 1462-2277 pMB1 replication origin: 2445-3064
pSpot3 vector

View More Images (2)

×
T7 promoter: 1-19 Lac operon: 19-46 RBS: 64-80 MCS: 86-140 Spot-Tag®: 141-176 Stop codon (TAA): 177-179 rrnB terminator: 361-507 Ampicillin resistance gene: 955-1815 pRB322 replication origin: 1970-2589 Lac operon: 2895-2922

Vector Type

bacterial expression vector

Promoter

T7

Reporter

Spot-Tag® C-terminal

Selection

ampicillin

Sizes
-_- / -_-

Cat No. ev-3
pSpot3 vector
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

T7 promoter: 1-19 Lac operon: 19-46 RBS: 64-80 MCS: 86-140 Spot-Tag®: 141-176 Stop codon (TAA): 177-179 rrnB terminator: 361-507 Ampicillin resistance gene: 955-1815 pRB322 replication origin: 1970-2589 Lac operon: 2895-2922

Multiple cloning site (MCS)
pSpot4 vector

View More Images (2)

×
T7 promoter: 1-19 Lac operon: 18-46 RBS: 64-80 Start codon (ATG): 88-90 Spot-Tag®: 91-126 MCS: 127-172 rrnB terminator: 364-510 Ampicillin resistance gene: 958-1818 pRB322 replication origin: 1973-2592 Lac operon: 2898-2925

Vector Type

bacterial expression vector

Promoter

T7

Reporter

Spot-Tag® N-terminal

Selection

ampicillin

Sizes
-_- / -_-

Cat No. ev-4
pSpot4 vector
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

T7 promoter: 1-19 Lac operon: 18-46 RBS: 64-80 Start codon (ATG): 88-90 Spot-Tag®: 91-126 MCS: 127-172 rrnB terminator: 364-510 Ampicillin resistance gene: 958-1818 pRB322 replication origin: 1973-2592 Lac operon: 2898-2925

Multiple cloning site (MCS)
pSpot6 vector

View More Images (2)

×
TPI1 promoter: 7239-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 47-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 CEN replication origin: 3398-3916 Ampicillin resistance gene: 4048-4908 pRB322 replication origin: 5063-5682

Vector Type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® C-terminal

Selection

leucin (S. cerevisiae) ampicillin (E. coli)

Sizes
-_- / -_-

Cat No. ev-6
pSpot6 vector
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

TPI1 promoter: 7239-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 47-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 CEN replication origin: 3398-3916 Ampicillin resistance gene: 4048-4908 pRB322 replication origin: 5063-5682

Multiple cloning site (MCS)
pSpot7 vector

View More Images (2)

×
TPI1 promoter: 7232-9 MCS: 14-73 Spot-Tag®: 73-108 Stop codon (TAA): 109-111 T7 primer site: 223-241 Leucin marker (LEU2): 1557-2663 CEN replication origin: 3391-3909 Ampicillin resistance gene: 4041-4901 pRB322 replication origin: 5056-5675

Vector Type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® N-terminal

Selection

leucin (S. cerevisiae) ampicillin (E. coli)

Sizes
-_- / -_-

Cat No. ev-7
pSpot7 vector
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

TPI1 promoter: 7232-9 MCS: 14-73 Spot-Tag®: 73-108 Stop codon (TAA): 109-111 T7 primer site: 223-241 Leucin marker (LEU2): 1557-2663 CEN replication origin: 3391-3909 Ampicillin resistance gene: 4041-4901 pRB322 replication origin: 5056-5675

Multiple cloning site (MCS)
pSpot8 vector

View More Images (2)

×
TPI1 promoter: 8914-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 53-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 2µ replication origin: 3396-4865 Ampicillin resistance gene: 5723-6583 pRB322 replication origin: 6738-7357

Vector Type

yeast expression vector

Promoter

TPI1

Reporter

Spot-Tag® C-terminal

Selection

leucin (S. cerevisiae) ampicillin (E. coli)

Sizes
-_- / -_-

Cat No. ev-8
pSpot8 vector
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

TPI1 promoter: 8914-9 Start codon (ATG): 10-12 Spot-Tag®: 13-48 MCS: 53-112 T7 primer site: 230-248 Leucin marker (LEU2): 1564-2670 2µ replication origin: 3396-4865 Ampicillin resistance gene: 5723-6583 pRB322 replication origin: 6738-7357

Multiple cloning site (MCS)
pSpot-Tag-Actin vector

View More Images (2)

×
HeLa cells were transfected with the Spot-Tag®-Actin plasmid overnight, fixed, stained with Spot-Label ATTO594 (product code eba594), and imaged using 40X objective. Spot-Tag®-Actin filaments in red, cell nuclei in blue (DAPI). Scale bar 10 µm.

Vector Type

Mammalian expression vector

Promoter

CMV

Reporter

Spot-Tag® N-terminal

Selection

Kan/Neo

Sizes
-_- / -_-

Cat No. ev-31
pSpot-Tag-Actin vector
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

HeLa cells were transfected with the Spot-Tag®-Actin plasmid overnight, fixed, stained with Spot-Label ATTO594 (product code eba594), and imaged using 40X objective. Spot-Tag®-Actin filaments in red, cell nuclei in blue (DAPI). Scale bar 10 µm.

CMV promoter: 508-589 Spot-Tag®-actin: 613-1776 Spot-Tag®: 616-651 SV40 terminator: 1799-2043 Kanamycin resistance gene: 3005-3805 pMB1 replication origin: 4400-5019
Spot-Cap® and Peptide

$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.$
View More Images (6)

×
Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.
$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.$

Application

Protein purification

Conjugate

Agarose

Specificity

Spot-Tag^®

Sizes
-_- / -_-

Cat No. eca-ep
$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.$

Spot-Cap® and Peptide
-_- / -_-
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.$

Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in gravity flow column format. Elution 1-6: 6 fractions à 2 CVs Spot-peptide (100 μM) in PBS.

Workflow of Spot-Cap purification.

Spot-Cap has a higher selectivity than anti-DYKDDDDK resin. While Spot-Cap is optimized for minimal contamination of host cell proteins, the anti-DYKDDDDK resin binds significant amounts of mammalian proteins. Incubation of control HEK293T cell lysate expressing no tagged protein with Spot-Cap or anti-DYKDDDDK resin. Elution with 100 μM Spot-peptide or 0.1 g/L DYKDDDDK-peptide. Similar results were obtained for yeast, bacteria, and insect cell lysates (data not shown).

$The elution efficiency of Spot-Cap is constant even after 4 regeneration cycles. Elution of Spot-tagged PCNA with Spot-peptide. R0: Reference (elution fraction of Spot-PCNA without regeneration); R1-R4: Elution fraction of Spot-PCNA after 1, 2, 3, and 4 regeneration cycles with 100 mM glycine pH 2.0.$

The elution efficiency of Spot-Cap is constant even after 4 regeneration cycles. Elution of Spot-tagged PCNA with Spot-peptide. R0: Reference (elution fraction of Spot-PCNA without regeneration); R1-R4: Elution fraction of Spot-PCNA after 1, 2, 3, and 4 regeneration cycles with 100 mM glycine pH 2.0.

$Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in batch format. Elution: 6 fractions à 2 BVs Spot-peptide (100 μM) in PBS.$

Purification of Spot-tagged GFP with Spot-Cap from HEK293T cell lysate in batch format. Elution: 6 fractions à 2 BVs Spot-peptide (100 μM) in PBS.

Application of Spot-tag related products.

Spot-Traps

-_- / -_-

-_- / -_-

Spot-Labels

-_- / -_-

-_- / -_-

Spot VHH, recombinant binding protein

-_- / -_-

-_- / -_-

pSpot2 vector

-_- / -_-

-_- / -_-

Spot-Cap®

-_- / -_-

-_- / -_-

pSpot5 vector

-_- / -_-

-_- / -_-

Spot-peptide

-_- / -_-

-_- / -_-

pSpot2 GFPSpot-Tag vector

-_- / -_-

-_- / -_-

pSpot3 vector

-_- / -_-

-_- / -_-

pSpot4 vector

-_- / -_-

-_- / -_-

pSpot6 vector

-_- / -_-

-_- / -_-

pSpot7 vector

-_- / -_-

-_- / -_-

pSpot8 vector

-_- / -_-

-_- / -_-

pSpot-Tag-Actin vector

-_- / -_-

-_- / -_-

Spot-Cap® and Peptide

-_- / -_-

-_- / -_-

Products derived from