SOAT1 fusion protein Ag30956 SDS-PAGE

Daudi cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

K-562 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DU 145 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 MCF-7 cells were stained with 0.2ug STAT5A/B antibody (13179-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Various lysates were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

IP result of anti-STAT5A/B (IP:13179-1-AP, 4ug; Detection:13179-1-AP 1:5000) with 3T3-L1 cells lysate 1360 ug.

IP result of anti-STAT5A/B (IP:13179-1-AP, 4ug; Detection:13179-1-AP 1:5000) with K-562 cells lysate 2800 ug.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13179-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Non-treated TF-1 and GM-CSF (HZ-1002) treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80115-1-RR (Phospho-STAT5A (Tyr694) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

Various lysates were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

G66427-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G66427-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Various lysates were subjected to SDS PAGE followed by western blot with 12071-1-AP (STAT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-STAT5A/B(IP:12071-1-AP, 4ug; Detection:12071-1-AP 1:4000) with HeLa cells lysate 1720 ug.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12071-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of HepG2 cells using 12071-1-AP (STAT5A/B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 68000-1-lg (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

Various lysates were subjected to SDS PAGE followed by western blot with 51072-2-AP (STAT5B-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF (red) were intracellularly stained with 0.06 ug CoraLite®488 Anti-Human Phospho-STAT5A (Tyr694) (CL488-80115, Clone:2P21), or 0.06 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.