Boiled and unboiled mouse heart lysates were subjected to SDS PAGE followed by western blot with 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SERCA2,ATP2A2 (CL488-67248, Clone:1F11A11) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).