1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RB1 (CL488-67521, Clone:1A2A6) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.