Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28731-1-AP (Phospho-AKT (Ser473) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28731-1-AP (Phospho-AKT (Ser473) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 29163-1-AP (Phospho-AKT (Thr308) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0115 (AKT IHC Kit).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0115 (AKT IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0115 (AKT IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0115 (AKT IHC Kit).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using KHC0115 (AKT IHC Kit).

Immunohistochemical analysis of paraffin-embedded Jurkat (left) and calyculin A treated Jurkat (right) cells slide using KHC0293 (Phospho-AKT (Ser473) IHC Kit).

AKT fusion protein Ag26642 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 21641-1-AP (AKT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21641-1-AP (AKT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

K-562 cells were subjected to SDS PAGE followed by western blot with 13739-1-AP (RACGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IP result of anti-RACGAP1 (IP:13739-1-AP, 4ug; Detection:13739-1-AP 1:500) with mouse testis tissue lysate 4000ug.

Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 10x lens)..

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 40x lens)..

Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 24195-1-AP (PREX2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 30042-1-AP (RACGAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30042-1-AP (RACGAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RACGAP1 antibody (30042-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).