Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Paxillin antibody (10029-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).
Immunofluorescent analysis of -20℃ Ethanol fixed HepG2 cells using 10029-1-Ig (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin). DAPI (blue).
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP result of anti-Paxillin (IP:10029-1-Ig, 3ug; Detection:10029-1-Ig 1:1000) with mouse brain tissue lysate 7500ug.
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 40x lens).
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (Paxillin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10029-1-Ig (Paxillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Various lysates were subjected to SDS PAGE followed by western blot with 32083-1-AP (Paxillin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.