Various lysates were subjected to SDS PAGE followed by western blot with 29115-1-AP (PTH1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).