HeLa cells were subjected to SDS PAGE followed by western blot with 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).