Various lysates were subjected to SDS PAGE followed by western blot with 14188-1-AP (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IP result of anti-PKC Delta (IP:14188-1-AP, 4ug; Detection:14188-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14188-1-AP (PKC Delta antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:800) with A431 cells lysate 2640 ug.

IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug.

Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Delta (19132-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently.

Various lysates were subjected to SDS PAGE followed by western blot with 11254-1-AP (GLRX3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11254-1-AP (GLRX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29123-1-AP (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated and PMA treated HL-60 cells were subjected to SDS PAGE followed by western blot with 29123-1-AP (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PKC Alpha (Thr638) antibody (29123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28926-1-AP (Phospho-PKC Alpha (Ser657) antibody) at dilution of 1:1500 incubated at room temperature for 1 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PKC Alpha (Ser657) antibody (28926-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10860-1-AP (PKC zeta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PKC Zeta antibody (10860-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Non-treated Jurkat, phosphatase inhibitor treated and λ phosphatase treated Jurkat cells were subjected to SDS PAGE followed by western blot with 29562-1-AP (Phospho-PKC Delta (Ser359) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated A431 cells using Phospho-PKC Delta (Ser359) antibody (29562-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).