100 ul human peripheral blood were surface stained with 20.00 ul PE Anti-Human CD34 (PE-65183, Clone: QBEnd-10) and 5 ul APC Anti-Human CD45 (APC-65109, Clone: HI30), and then treated with red blood cell lysis buffer. Cells were not fixed.
1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.20 ug PE-Anti-Mouse Ly-6G (Gr-1) (PE-65140, clone RB6-8C5) (red) or 0.20 ug PE-rat IgG2b isotype control (blue). Cells were not fixed.
1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug PE Mouse IgG2a Isotype Control (PE-65208, Clone: C1.18.4) (black) or 0.5 ug PE Anti-Human CD45 (PE-65064, Clone: F10-89-4) (red). Cells were not fixed.