Results

Neurofibromin 1 Recombinant antibody

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Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

CloneNo.

240540F2

Sizes
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Cat No. 83620-5-RR
Neurofibromin 1 Recombinant antibody
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Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

1x10^6 HeLa cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Biolayer interferometry (BLl) kinetic assays of 83620-5-RR against Human Neurofibromin 1 were performed. The affinity constant is below 1 pM.
Neurofibromin 1 Polyclonal antibody

KO/KD Validated

6 Publications

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Various lysates were subjected to SDS PAGE followed by western blot with 27249-1-AP (Neurofibromin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Sizes
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Cat No. 27249-1-AP
Neurofibromin 1 Polyclonal antibody
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Various lysates were subjected to SDS PAGE followed by western blot with 27249-1-AP (Neurofibromin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27249-1-AP (Neurofibromin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27249-1-AP (Neurofibromin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27249-1-AP (Neurofibromin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Neurofibromin 1 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00570-1	83620-2-PBS	83620-1-PBS	Neurofibromin 1	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00570-2	83620-5-PBS	83620-4-PBS	Neurofibromin 1	Human	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00570-3	83620-2-PBS	83620-3-PBS	Neurofibromin 1	Human	Cytometric bead array	CBA: 0.313-40 ng/mL

IHCeasy NF1 Ready-To-Use IHC Kit

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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC1057 (NF1 IHC Kit).

Primary Ab Type

Rabbit Polyclonal

Reactivity

human, mouse, rat

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Rabbit

Sizes
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Cat No. KHC1057
IHCeasy NF1 Ready-To-Use IHC Kit
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The search results gallery shows a selection of our total image library
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC1057 (NF1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using KHC1057 (NF1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using KHC1057 (NF1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC1057 (NF1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using KHC1057 (NF1 IHC Kit).
Neurofibromin Fusion Protein

Enlarge Image

Species

human

Source

E. coli-derived, PET30a

Tag

6*His

Format

Powder

Sizes
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Cat No. Ag25799
Neurofibromin Fusion Protein
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Neurofibromin fusion protein Ag25799 SDS-PAGE
NFIA Polyclonal antibody

4 Publications

Reviews (1)

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Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Sizes
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Cat No. 11750-1-AP
NFIA Polyclonal antibody
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

A431 cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP Result of anti-NFIA (IP:11750-1-AP, 4ug; Detection:11750-1-AP 1:500) with A431 cells lysate 2000ug.
NFIA Monoclonal antibodies (3A6D7)

(Parent antibody plus 1 derived formats/conjugates)

KO/KD Validated

1 Publications

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

ELISA, IP, WB

Conjugation

Unconjugated

CloneNo.

3A6D7
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Unconjugated parent antibody
Cat No. 66999-1-Ig

Other conjugate/format antibodies
Cat No. 66999-1-PBS
NFIA Monoclonal antibodies (3A6D7) (2 total products)
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IHCeasy NFIA Ready-To-Use IHC Kit

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Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using KHC1693 (NFIA IHC Kit).

Primary Ab Type

Rabbit Polyclonal

Reactivity

human, mouse, rat

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Rabbit

Sizes
-_- / -_-

Cat No. KHC1693
IHCeasy NFIA Ready-To-Use IHC Kit
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×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using KHC1693 (NFIA IHC Kit).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC1693 (NFIA IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using KHC1693 (NFIA IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC1693 (NFIA IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using KHC1693 (NFIA IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using KHC1693 (NFIA IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using KHC1693 (NFIA IHC Kit).
NFIB Polyclonal antibody

KO/KD Validated

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Various lysates were subjected to SDS PAGE followed by western blot with 29898-1-AP (NFIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Sizes
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Cat No. 29898-1-AP
NFIB Polyclonal antibody
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×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Various lysates were subjected to SDS PAGE followed by western blot with 29898-1-AP (NFIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFIB antibody (29898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NFIB (29898-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29898-1-AP (NFIB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFIB antibody (29898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
NFIA E.Coli expressed Tagged-proteins

Species

human

Source

PET28a, PGEX-4T

Tag

6*His, GST
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Cat No. Ag17873, Ag2346
NFIA E.Coli expressed Tagged-proteins (2 total products)
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NFIB E.Coli expressed Tagged-proteins

Species

human

Source

PET28a, PGEX-4T

Tag

6*His, GST
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Cat No. Ag31991, Ag31946
NFIB E.Coli expressed Tagged-proteins (2 total products)
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