Various lysates were subjected to SDS PAGE followed by western blot with 27883-1-AP (MDM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB result of MDM2 antibody (27883-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDM2 transfected A431 cells.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MDM2 (27883-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated U2OS cells using MDM2 antibody (27883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
U2OS cells were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28356-1-AP (MTBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).