HEK-293 cells were subjected to SDS PAGE followed by western blot with 51094-1-AP (MEK6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Non-treated HEK-293 cells, UV treated HEK-293 cells, non-treated HeLa cells and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 28903-1-AP (Phospho-MEK6 (Ser207) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.