IHCeasy BCL2 Ready-To-Use IHC Kit
BCL2 Ready-to-use reagent kit for IHC.
Cat no : KHC0012
Synonyms
Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2
Validation Data Gallery
Product Information
KHC0012 is a ready-to-use IHC kit for staining of BCL2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy BCL2 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, BCL-2, Oncogene B cell leukemia 2 |