RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

UV treated and non-treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80086-1-RR (Phospho-JUN (Ser73) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody (66009-1-Ig) as loading control.

Various lysates were subjected to SDS PAGE followed by western blot with 24909-1-AP (JUN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24909-1-AP (JUN Antibody) at dilution of 1:50 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24909-1-AP (JUN Antibody) at dilution of 1:50 (under 40x lens).

Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using JUN antibody (24909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human JUN (24909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 JUN antibody (CL488-24909) at dilution of 1:200, CL594-phalloidin (red).

RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66313-1-PBS in a different storage buffer formulation.

UV treated and non-treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80086-1-RR (Phospho-JUN (Ser73) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 80086-1-PBS in a different storage buffer formulation.

Non-treated NIH/3T3 and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28891-1-AP (Phospho-JUN (Ser73) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunofluorescent analysis of (4% PFA) fixed UV treated and non-treated NIH/3T3 cells using Phospho-JUN (Ser73) antibody (28891-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22114-1-AP (JUN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 22114-1-AP (JUN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

AP1,JUN,P39 fusion protein Ag17419 SDS-PAGE