Purity of recombinant human IL-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human IL-4 (HZ-1004) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-4 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.07-0.4 ng/mL.

Purity of recombinant human IL-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-4 (HZ-1004-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant IL-4 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.07-0.4 ng/mL.

Recombinant human IL-4 (Cat.NO. HZ-1004) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-4 (0.5 ng/mL HZ-1004) is neutralized by mouse anti-human IL-4 monoclonal antibody 69005-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 200-500 ng/mL.

IL-4 fusion protein Ag9940 SDS-PAGE

WB result of STAT6 antibody (51073-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected NIH/3T3 cells.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1151 (STAT6 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using KHC1151 (STAT6 IHC Kit).