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115 product results for "IL-2"

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HumanKine® recombinant human IL-2 protein

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

26 Publications

Reviews (1)

View More Images (2)

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Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.05-0.35 ng/mL

Expression

HEK293

Reactivity

human, mouse, rat

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1015
HumanKine® recombinant human IL-2 protein
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Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

recombinant human IL-2 (HZ-1015) stimulates dose-dependent proliferation of the CTLL-2 (mouse cytotoxic T) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. CTLL-2 cells were starved for 5 hours before treatment with increasing concentrations of GMP recombinant human IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.05-0.35 ng/mL.
HumanKine® recombinant human IL-2 protein- GMP grade

ENDOTOXIN-FREE

ANIMAL-COMPONENT FREE

1 Publications

View More Images (2)

×
Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Activity

0.05-0.35 ng/mL

Expression

HEK293

Reactivity

human, mouse

Purity

>95%

Sizes
-_- / -_-

Cat No. HZ-1015-GMP
HumanKine® recombinant human IL-2 protein- GMP grade
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×

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Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-2 (HZ-1015-GMP) induces dose-dependent proliferation of the CTLL2 (mouse cytotoxic T cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. CTLL2 cells were treated with increasing concentrations of GMP recombinant IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.05-0.35 ng/mL.
IL2 Recombinant antibodies (240416H3)

(Parent antibody plus 1 derived formats/conjugates)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB

Conjugation

Unconjugated

CloneNo.

240416H3
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Unconjugated parent antibody
Cat No. 83558-5-RR

Other conjugate/format antibodies
Cat No. 83558-5-PBS
IL2 Recombinant antibodies (240416H3) (2 total products)
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IL-2 Polyclonal antibody

12 Publications

Reviews (1)

View More Images (3)

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PMA, A23187 and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 26156-1-AP (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA, Cell treatment

Sizes
-_- / -_-

Cat No. 26156-1-AP
IL-2 Polyclonal antibody
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PMA, A23187 and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 26156-1-AP (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26156-1-AP (Il2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26156-1-AP (Il2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IL-2 Monoclonal antibodies (2F11C5)

(Parent antibody plus 3 derived formats/conjugates)

8 Publications

Reviews (1)

Host/IsoType

Mouse/IgG2a

Reactivity

human, mouse, pig

Applications

ELISA, IF-P, IHC, WB

Conjugation

CoraLite® Plus 488, CoraLite®594, Unconjugated

CloneNo.

2F11C5
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Unconjugated parent antibody
Cat No. 60306-1-Ig

Other conjugate/format antibodies
Cat No. CL594-60306, 60306-1-PBS, CL488-60306
IL-2 Monoclonal antibodies (2F11C5) (4 total products)
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IL-2 Recombinant antibodies (240358B1)

(Parent antibody plus 1 derived formats/conjugates)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

Cytometric bead array, ELISA, WB

Conjugation

Unconjugated

CloneNo.

240358B1
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Unconjugated parent antibody
Cat No. 83547-3-RR

Other conjugate/format antibodies
Cat No. 83547-3-PBS
IL-2 Recombinant antibodies (240358B1) (2 total products)
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Mouse IL-2 ELISA Kit

12 Publications

Reviews (1)

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

0.5 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE10004
Mouse IL-2 ELISA Kit
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IL-2 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP00533-1	83547-1-PBS	83547-2-PBS	IL-2	Mouse	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00533-2	83547-2-PBS	83547-3-PBS	IL-2	Mouse	Cytometric bead array	CBA: 0.313-40 ng/mL
MP00533-3	98055-1-PBS	83547-1-PBS	IL-2	Mouse	Sandwich ELISA	sELISA: 6.25-400 pg/mL
MP00535-1	82626-2-PBS	82626-3-PBS	IL-2	Rat	Cytometric bead array	CBA: 0.156-20 ng/mL
MP00535-2	82626-2-PBS	82626-4-PBS	IL-2	Rat	Cytometric bead array	CBA: 0.156-20 ng/mL
MP00535-3	82626-2-PBS	82626-5-PBS	IL-2	Rat	Sandwich ELISA	sELISA: 15.6-1000 pg/mL
MP00545-1	83558-2-PBS	83558-3-PBS	IL-2	Human	Cytometric bead array	CBA: 0.625-40 ng/mL
MP00545-2	83558-2-PBS	83558-1-PBS	IL-2	Human	Cytometric bead array	CBA: 0.625-40 ng/mL
MP00545-3	83558-4-PBS	83558-1-PBS	IL-2	Human	Cytometric bead array	CBA: 0.625-40 ng/mL

Human IL-2 ELISA Kit

7 Publications

View More Images (2)

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

3.6 pg/mL

Range

15.6-1000 pg/mL

Sizes
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Cat No. KE00017
Human IL-2 ELISA Kit
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IL-2 Polyclonal antibody

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Untreated, PMA, A23187 and Brefeldin A treated were subjected to SDS PAGE followed by western blot with 31506-1-AP (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

WB, ELISA

Sizes
-_- / -_-

Cat No. 31506-1-AP
IL-2 Polyclonal antibody
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×

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Untreated, PMA, A23187 and Brefeldin A treated were subjected to SDS PAGE followed by western blot with 31506-1-AP (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IL-2 Recombinant antibodies (240358A2)

(Parent antibody plus 1 derived formats/conjugates)

Host/IsoType

Rabbit/IgG

Reactivity

mouse

Applications

ELISA, FC (Intra)

Conjugation

Unconjugated

CloneNo.

240358A2
-_- / -_-

Unconjugated parent antibody
Cat No. 98055-1-RR

Other conjugate/format antibodies
Cat No. 98055-1-PBS
IL-2 Recombinant antibodies (240358A2) (2 total products)
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IL-2 Recombinant antibodies (240416E3)

(Parent antibody plus 1 derived formats/conjugates)

Host/IsoType

Rabbit/IgG

Reactivity

human

Applications

FC (Intra)

Conjugation

Unconjugated

CloneNo.

240416E3
-_- / -_-

Unconjugated parent antibody
Cat No. 98053-1-RR

Other conjugate/format antibodies
Cat No. 98053-1-PBS
IL-2 Recombinant antibodies (240416E3) (2 total products)
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Mouse IL-2 ELISA Kit

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant

Sensitivity

4.5 pg/mL

Range

31.25-2000 pg/mL

Sizes
-_- / -_-

Cat No. KE10100
Mouse IL-2 ELISA Kit
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Mouse IL-2 ELISA Kit

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Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant

Sensitivity

2.3 pg/mL

Range

12.5-800 pg/mL

Sizes
-_- / -_-

Cat No. KE10126
Mouse IL-2 ELISA Kit
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Standard Curve of KE10126 Mouse IL-2 ELISA Kit

Standard Curve of KE10126 Mouse IL-2 ELISA Kit
Rat IL-2 ELISA Kit

1 Publications

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatants

Sensitivity

8.3 pg/mL

Range

31.25-2000 pg/mL

Sizes
-_- / -_-

Cat No. KE20002
Rat IL-2 ELISA Kit
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Standard curve of Rat IL-2 ELISA Kit KE20002.
IL2 E.Coli expressed Tagged-proteins

Species

human, mouse

Source

PET28a, PGEX-4T

Tag

6*His, GST
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Cat No. Ag24086, Ag24079, Ag10199
IL2 E.Coli expressed Tagged-proteins (3 total products)
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IL-2 Polyclonal antibody

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1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ), and 0.5 ug CoraLite®594 Anti-Rat CD8a (OX-8) (CL594-65180, Clone: OX-8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Host/IsoType

Rabbit/IgG

Reactivity

rat

Applications

ELISA, FC (Intra)

Sizes
-_- / -_-

Cat No. 30199-1-AP
IL-2 Polyclonal antibody
-_- / -_-
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1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ), and 0.5 ug CoraLite®594 Anti-Rat CD8a (OX-8) (CL594-65180, Clone: OX-8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IL2 Recombinant antibodies (240264C7)

(Parent antibody plus 6 derived formats/conjugates)

Host/IsoType

Rabbit/IgG

Reactivity

rat

Applications

FC (Intra)

Conjugation

CoraLite® Plus 405, CoraLite® Plus 488, CoraLite® Plus 647, CoraLite® Plus 750, CoraLite®594, Unconjugated

CloneNo.

240264C7
-_- / -_-

Unconjugated parent antibody
Cat No. 98046-1-RR

Other conjugate/format antibodies
Cat No. CL488-98046, CL594-98046, 98046-1-PBS, CL405-98046, CL647-98046, CL750-98046
IL2 Recombinant antibodies (240264C7) (7 total products)
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Rat IL-2 ELISA Kit

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant

Sensitivity

2.2 pg/mL

Range

15.6-1000 pg/mL

Sizes
-_- / -_-

Cat No. KE20025
Rat IL-2 ELISA Kit
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Standard Curve of KE20025 Rat IL-2 ELISA Kit
Rat IL-2 ELISA Kit

Enlarge Image

Assay Type

Sandwich

Sample Type

Serum, Plasma, Cell culture supernatant

Sensitivity

0.5 pg/mL

Range

7.8-500 pg/mL

Sizes
-_- / -_-

Cat No. KE20019
Rat IL-2 ELISA Kit
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Anti-Human CD25 (BC96) Recombinant antibodies

(Parent antibody plus 1 derived formats/conjugates)

Host/IsoType

Mouse/IgG2a

Reactivity

human

Applications

FC

Conjugation

Unconjugated

CloneNo.

BC96
-_- / -_-

Unconjugated parent antibody
Cat No. 65620-1-MR

Other conjugate/format antibodies
Cat No. 65620-1-PBS
Anti-Human CD25 (BC96) Recombinant antibodies (2 total products)
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HumanKine® recombinant human IL-2 protein

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HumanKine® recombinant human IL-2 protein- GMP grade

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IL2 Recombinant antibodies (240416H3)

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-_- / -_-

IL-2 Polyclonal antibody

-_- / -_-

-_- / -_-

IL-2 Monoclonal antibodies (2F11C5)

-_- / -_-

-_- / -_-

IL-2 Recombinant antibodies (240358B1)

-_- / -_-

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Mouse IL-2 ELISA Kit

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IL-2 Matched Antibody PairsNew

Human IL-2 ELISA Kit

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IL-2 Polyclonal antibody

-_- / -_-

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IL-2 Recombinant antibodies (240358A2)

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IL-2 Recombinant antibodies (240416E3)

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Mouse IL-2 ELISA Kit

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Mouse IL-2 ELISA Kit

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Rat IL-2 ELISA Kit

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IL2 E.Coli expressed Tagged-proteins

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IL-2 Polyclonal antibody

-_- / -_-

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IL2 Recombinant antibodies (240264C7)

-_- / -_-

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Rat IL-2 ELISA Kit

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Rat IL-2 ELISA Kit

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Anti-Human CD25 (BC96) Recombinant antibodies

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Products derived from

IL-2 Matched Antibody Pairs^New