Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL-18 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (10663-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
WB result of Pre-IL18 antibody (10663-1-AP, 1:4000) with si-Control and si-Pre-IL18 transfected HeLa cells..
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human IL-18 (10663-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP result of anti-IL-18 (IP:10663-1-AP, 4ug; Detection:10663-1-AP 1:5000) with HeLa cells lysate 1360 ug.
IL-18 Recombinant antibodies (1H14)
(Parent antibody plus 1 derived formats/conjugates)