Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human IFN gamma (HZ-1301) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.

Proteintech IFN gamma (HZ-1301) demonstrates a 3-fold lower EC50 and equivalent dose-dependent inhibition of proliferation to a leading competitor. Recombinant human IFN gamma dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.

Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IFN gamma (HZ-1301-GMP) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of GMP recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.02-0.14 ng/mL.

PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 15365-1-AP (IFN Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15365-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30293-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30293-1-AP (IFN Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IFN Gamma (30293-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35), and 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Standard curve of Rat IFN-gamma ELISA Kit KE20007.

PMA, Ionomycin and Brefeldin A treated CTLL-2 cells were subjected to SDS PAGE followed by western blot with 29788-1-AP (IFN gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Non-treated, PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 28722-1-AP (IFNG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

G69007-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G69007-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G69007-1-5C was used to stain PBMC under 4hr PMA + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

Recombinant human IFN-gamma (Cat.NO. HZ-1301) inhibits HT-29 cell line (human colorectal adenocarcinoma cell line) proliferation in the prescence of 0.5 ng/mL TNF alpha in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IFN-gamma (5 ng/mL HZ-1301 axis) is neutralized by mouse anti-human IFN-gamma monoclonal antibody 69007-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 100-500 ng/mL. The NeutraControl mouse anti-human IFN-gamma monoclonal antibody 69507-1-Ig could recognize human IFN-gamma but could not neutralize human IFN-gamma in the range of 0.00244-40 μg/mL (green curve, refer to top X-right Y axis).

Indirect ELISA was carried out by coating recombinant Human IFN gamma (Cat.NO. HZ-1301) at 70 ng/well followed by blocking and adding serial diluted IFN gamma antibody 69007-1-Ig and 69507-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.