Purity of recombinant human BMP-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human BMP-2 (HZ-1128-GMP) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-2 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 7.5-37.5 ng/ml.

Purity of recombinant human FGF-8b was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human FGF-8b (HZ-1103-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved in 0.02% FBS with 1 ug/mL heparin during treatment with increasing concentrations of recombinant human FGF-8b for 72hrs. Activity determination was conducted in triplicate on a validated bioassay. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 10-60 ng/mL.
 

Purity of recombinant human GM-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human GM-CSF (HZ-1002-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant GM-CSF for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.08-0.8 ng/mL​.

Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-2 (HZ-1015-GMP) induces dose-dependent proliferation of the CTLL2 (mouse cytotoxic T cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. CTLL2 cells were treated with increasing concentrations of GMP recombinant IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.05-0.35 ng/mL.

Purity of GMP-grade recombinant human IL-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP recombinant human IL-7 (HZ-1281-GMP) stimulates dose-dependent proliferation of the 2E8 murine B lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.1-1.4 ng/mL.
 

Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human GMP TGF beta 1 (HZ-1011-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant GMP TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.01-0.17 ng/mL.

Purity of recombinant human VEGF165 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP recombinant human VEGF165 (HZ-1038-GMP) induces dose-dependent luciferase production in a HEK293T reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293T reporter cells were treated with increasing concentrations of recombinant VEGF165 for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.3-3.75 ng/mL.

GMP Recombinant human VEGF165 (HZ-1038-GMP) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of GMP recombinant VEGF165 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.3-3.75 ng/mL.

Purity of recombinant human Activin A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human Activin A (HZ-1138-GMP) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of GMP recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.5-3.5 ng/ml

Purity of recombinant human BDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP BDNF (HZ-1335-GMP) stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 4-20 ng/mL.
 

Purity of recombinant human beta NGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
 

GMP recombinant human beta NGF (HZ-1222-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. TF-1 cells were treated with increasing concentration of recombinant beta NGF for 72 hours. Activity determination was conducted in triplicate on a validated bioassay. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.00 ng/mL.

Purity of recombinant human BMP-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human BMP-4 (HZ-1045-GMP) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 1.5-9 ng/ml.

Purity of recombinant human BMP-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
 

Recombinant human BMP-7(HZ-1229-GMP) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-7 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 50-275 ng/ml.
 

Purity of GMP recombinant human Cystatin C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant Human GMP Cystatin C (HZ-1211-GMP) inhibits papain protease activity in a dose-dependent manner. Papain protease activity was measured by colorimetric assay using L-BAPA as the substrate. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range form 0.5-2.6 µg/mL.

Purity of GMP-grade recombinant human EGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human EGF (HZ-1326-GMP) induces dose-dependent proliferation of the 4MBr-5 (monkey epithelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. 4MBr-5 cells were treated with increasing concentrations of recombinant EGF for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.1-0.6 ng/mL.

Purity of GMP-grade recombinant human EPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human EPO (HZ-1168-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant EPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.2-3.0 ng/mL
 

Purity of recombinant human FGF-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human GMP FGF-4 (HZ-1218-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-4 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated assay. The EC50 values range from 6-30 ng/mL.
 

Purity of GMP-grade recombinant human FGF-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP-grade recombinant human FGF-7 (HZ-1100-GMP) Stimulates dose-dependent proliferation of the 4MBr-5 Monkey epithelial cell line. Viable cell number was quantitatively assessed by PrestoBlue Cell Viability Reagent. 4MBr-5 ells were treated with increasing concentrations of recombinant human FGF-7 for 120 hours. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 4-20 ng/mL.

Purity of recombinant human FGFbasic-TS was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human FGFbasic-TS (HZ-1285-GMP) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of GMP recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.05-0.4 ng/mL.

GMP-grade Recombinant human FGFbasic-TS (HZ-1285-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF basic-TS for 72hrs in defined medium. Activity determination was conducted in triplicate on a validated bioassay. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.4-2.5 ng/mL.

Purity of GMP-grade recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP-grade recombinant human FLT3 Ligand (HZ-1151-GMP) stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitiatively assessed by PrestoBlue Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours.The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 value ranges from 0.4-3.0 ng/mL.

Purity of GMP-grade recombinant human G-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP-grade recombinant human G-CSF (HZ-1207-GMP) Stimulates dose-dependent proliferation of the M-NFS-60 Mouse Myeloid Leukemia indicator cells line. Viable cell number was quantitatively assessed by PrestoBlue Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant human G-CSF for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.009-0.05 ng/mL.

Purity of recombinant human GDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 3-18 ng/mL.