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Immunofluorescent analysis of (4% PFA) fixed human lung cancer using FlexAble HRP Antibody Labeling Kit for Human IgG (KFA110) and Tyramide-555(orange) to label endogenous human IgG in the tissue.
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Various cell lysates were subject to SDS PAGE followed by western blot with anti-HSPA5 antibody (66574-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).
Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Vimentin antibody (60330-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).
Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Beta Catenin antibody (66379-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).
Various cell lysates were subjected to SDS PAGE followed by western blot with anti-GORASP2 antibody (66627-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025).
WB of HeLa and HEK-293 Cell lysates (30 μg/lane): Various cell lysates were detected with anti-Beta Catenin 66379-1-Ig, Mouse IgG1, 0.44 μg) labeled with FlexAble HRP Kit (KFA025, 0.88 μL) (Lane 1 and Lane 2); Anti- GORASP2 (66627-1-Ig, Mouse IgG1, 0.18 μg) with FlexAble HRP Kit (KFA025, 0.36 μL) (Lane 3 and Lane 4); anti-Beta Catenin (66379-1-Ig, Mouse IgG1, 0.44 μg) labeled with FlexAble HRP Kit (KFA025, Linker 1.76 μL) with Quencher 0.88 μL, add 0.18 μg GORASP2 antibody as competitor antibody (Lane 5 and Lane 6). Primary antibody : Linker : Quencher=0.5 μg : 1 μL : 2 μL
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025). Antibody used at dilution of 1:400 under 10x & 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025). Antibody used at dilution of 1:400 under 10x & 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-ATP5O antibody (66696-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-594 (red). Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-Fus antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
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Various cell lysates were subjected to SDS PAGE followed by western blot with anti-MARS antibody (67739-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045).
Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Importin Alpha 5 antibody (67897-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045).
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 antibody (67169-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-594 (red). Cell nuclei are in blue.
Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-MAP2 antibody (17490-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-MAP2 antibody (17490-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, and anti-GFAP antibody (60190-1-AP, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-Fus antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
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Various cell lysates were subjected to SDS PAGE followed by western blot with anti-c-SRC antibody (60315-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2b (KFA065).
Various cell lysates were subjected to SDS PAGE followed by western blot with anti-ANP32B antibody (66160-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2b (KFA065).
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM20 antibody (66777-1-Ig) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2b (KFA065) and Tyramide-594 (red). Cell nuclei are in blue.
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More validation images available on the product page
Various cell lysates were subjected to SDS PAGE followed by western blot with anti-P53 antibody (10442-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005).
Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Lamin B1 antibody (12987-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005).
Various cell lysates were subjected to SDS PAGE followed by western blot with anti-Vimentin antibody (10366-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005).
Various cell lysates were subjected to SDS PAGE followed by western blot with anti-GAPDH antibody (10494-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005).
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide with anti-E-cadherin Antibody(20874-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005). Antibody used at a dilution of 1:1000 under 10x and 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide with anti-E-cadherin Antibody(20874-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005). Antibody used at a dilution of 1:1000 under 10x and 40x lens. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0) and DAB substrate was used for detection.
Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GORASP2 antibody (10598-1-AP) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-594 (red). Cell nuclei are in blue.
Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-MAP2 antibody (17490-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-MAP2 antibody (17490-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, and anti-GFAP antibody (60190-1-AP, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-Fus antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.
Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-Calbindin antibody (14479-1-AP, green) labeled with FlexAble HRP Antibody Labeling Kit for Rabbit IgG (KFA005) and Tyramide-488, anti-FUS antibody (68262-1-Ig, orange) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG1 (KFA025) and Tyramide-555, and anti-GFAP antibody (60190-1-Ig, magenta) labeled with FlexAble HRP Antibody Labeling Kit for Mouse IgG2a (KFA045) and Tyramide-650. Cell nuclei are in blue.