Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HeLa cells were subjected to SDS PAGE followed by western blot with 16289-1-AP (HOIP antibody at dilution of 1:100 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 30797-1-AP (RNF31 / HOIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

WB result of RNF31 / HOIP antibody (30797-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF31 / HOIP transfected HEK-293 cells.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 / HOIP antibody (30797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).