Various lysates were subjected to SDS PAGE followed by western blot with 10309-1-AP (GPRC5A,RAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10309-1-AP (GPRC5A,RAI3 Antibody) at dilution of 1:200 (under 10x lens).

1x10^6 HeLa cells were intracellularly stained with 0.4 ug GPRC5A,RAI3 Polyclonal antibody (10309-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 55133-1-AP (S1PR1/EDG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-S1PR1/EDG1 (IP:55133-1-AP, 4ug; Detection:55133-1-AP 1:300) with mouse brain tissue lysate 2640ug.

1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug APJ Polyclonal antibody (20341-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20341-1-AP (APJ antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed MDCK cells using GPR161 antibody (13398-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

mouse brain tissue was subjected to SDS PAGE followed by western blot with 13398-1-AP (GPR161 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

THP-1 cells were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

THP-1 cells were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 40x lens).

HEK-293 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of HepG2 cells, using MAS1L antibody 20080-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Various lysates were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

MCF-7 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25898-1-AP (CCR7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25898-1-AP (CCR7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 13990-1-AP (GRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-GRK2 (IP:13990-1-AP, 4ug; Detection:13990-1-AP 1:1000) with HL-60 cells lysate 3040ug.

1x10^6 HeLa cells were intracellularly stained with 0.4 ug GRK2 Polyclonal antibody (13990-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

mouse brain tissue and rat brain tissue were subjected to SDS PAGE followed by western blot with 27567-1-AP (GABBR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27567-1-AP (GABBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27567-1-AP (GABBR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).