Purity of recombinant human G-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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Recombinant human G-CSF (HZ-1207) stimulates dose-dependent proliferation of the M-NFS-60 Mouse Myeloid Leukemia indicator cells line. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant human G-CSF for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 value range is 0.009-0.05 ng/mL.

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Purity of GMP-grade recombinant human G-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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GMP-grade recombinant human G-CSF (HZ-1207-GMP) Stimulates dose-dependent proliferation of the M-NFS-60 Mouse Myeloid Leukemia indicator cells line. Viable cell number was quantitatively assessed by PrestoBlue Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant human G-CSF for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.009-0.05 ng/mL.

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Various lysates were subjected to SDS PAGE followed by western blot with 17185-1-AP (G-CSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human G-CSF (17185-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Various lysates were subjected to SDS PAGE followed by western blot with 30415-1-AP (G-CSF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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G-CSF fusion protein Ag11261 SDS-PAGE

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human placenta tissue were subjected to SDS PAGE followed by western blot with 18310-1-AP (G-CSFR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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