Various lysates were subjected to SDS PAGE followed by western blot with 22148-1-AP (ENDOG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP result of anti-ENDOG (IP:22148-1-AP, 4ug; Detection:22148-1-AP 1:1000) with mouse heart tissue lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22148-1-AP (ENDOG antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21523-1-AP (EXOG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.