Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human TGF beta 1 (HZ-1011) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.01-0.17 ng/mL.
 

Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

Purity of recombinant human TGF beta 3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human TGF beta 3 (HZ-1090) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.14-0.75 ng/mL

Purity of recombinant human Noggin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human Noggin (HZ-1118-GMP) inhibits dose-dependent induction of alkaline phosphatase production by BMP-4 in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human Noggin and 40 ng/mL of BMP-4 (HZ-1045) for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-15 ng/mL.

Purity of recombinant human BDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

BDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 100 ng/mL.
 

Proteintech BDNF (Cat no: HZ-1335) demonstrates equivalent fold induction and a greater than 5-fold increase in potency compared to leading competitors. BDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 100 ng/mL.

Differentiation of SHSY5Y cells into neurons using HumanKine BDNF in the presence of Retinoic acid. The data demonstrates significantly better neuronal differentiation using HumanKine BDNF compared to leading competitor in the market.

Purity of recombinant human FGF-8b was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

The activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Recombinant human FGF-8b (HZ-1218) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved in 0.02% FBS with 1 ug/mL heparin during treatment with increasing concentrations of recombinant human FGF-8b for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 10-60 ng/mL.
 

Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta
 Johannes Kepler Universitat)

Purity of recombinant human SHH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human SHH (HZ-1306) stimulates dose-dependent induction of alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. C3H10T1/2 cells were treated with increasing concentrations of recombinant human SHH for 120 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values are no more than 350 ng/mL.
 

Purity of recombinant human FGF-8b was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human FGF-8b (HZ-1103-GMP) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved in 0.02% FBS with 1 ug/mL heparin during treatment with increasing concentrations of recombinant human FGF-8b for 72hrs. Activity determination was conducted in triplicate on a validated bioassay. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 10-60 ng/mL.
 

Purity of recombinant human GDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
 

GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

Proteintech GDNF (HZ-1311) demonstrates a greater fold induction of proliferation compared to a leading competitor at an equivalent EC50. GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta
 Johannes Kepler Universitat)

Purity of GMP recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

Recombinant human GMP TGF beta 1 (HZ-1011-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant GMP TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.01-0.17 ng/mL.

Purity of recombinant human BDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP BDNF (HZ-1335-GMP) stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 4-20 ng/mL.
 

Purity of recombinant human GDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 3-18 ng/mL.

Purity of recombinant human Noggin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human Noggin (HZ-1118-GMP) inhibits dose-dependent induction of alkaline phosphatase production by BMP-4 in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human Noggin and 40 ng/mL of BMP-4 (HZ-1045) for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 3-15 ng/mL.

Purity of recombinant human TGF beta 3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human TGF beta 3 (HZ-1090-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of GMP recombinant TGF beta 3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is ≤ 0.75 ng/mL

Various lysates were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP sample and control were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:500. 4000ug mouse brain tissue lysate and 4ug antibodies were mixed in the IP experiment.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WB result of TH antibody (66334-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TH transfected SH-SY5Y cells.

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66334-1-Ig(TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with CL488-66240 (Beta Tubulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66240 (beta Tubulin antibody) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Beta Tubulin (CL488-66240, Clone:1D4A4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

WB result of VMAT2 antibody (20873-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VMAT2 transfected Jurkat cells.

SGC-7901 cells were subjected to SDS PAGE followed by western blot with 20873-1-AP (VMAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20873-1-AP (VMAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20873-1-AP (VMAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VMAT2 antibody (20873-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble Biotin Kit (KFA111) and streptavidin-PE dye. The cells are co-stained with anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with CoraLite® Plus 488 (CL488-65197). Note: Cells treated with Endogenous Biotin-Blocking Kit from Thermofisher.

Live A431 cells were immunostained with anti-EGFR (cetuximab biosimilar) labeled with FlexAble Biotin Antibody Labeling Kit for Human IgG (KFA111) and Streptavidin-ATTO594 (red). Nuclei are in cyan.​ Epifluorescence images were acquired with a 20x objective and post-processed.​

Live SKBR-3 cells immunostained with anti-HER2 (trastuzumab biosimilar) labeled with FlexAble Biotin Antibody Labeling Kit for Human IgG (KFA111) and Streptavidin-ATTO594 (red).  Nuclei are in blue.  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescent analysis of (4% PFA) fixed human lung cancer using FlexAble Biotin Antibody Labeling Kits for Human IgG and Streptavidin-594(red) to label endogenous human IgG in the tissue.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-CD147 antibody labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-ATTO594​ (yellow). Nuclei are stained with DAPI (blue).  Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow Cyotometry of PBMC.  1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-APC. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246).  Cells are not fixed, Monocytes are gated. 

Flow cytometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-PE.  The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246).  Cells are not fixed, Monocytes are gated.

Flow cytometry of PBMC. 1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-PE.  The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246).  Cells are not fixed, Monocytes are gated.

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and used at a dilution of 1:400 (under 10x lens & 40xlens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using anti-Cytokeratin 5 antibody (66727-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and used at a dilution of 1:400 (under 10x lens & 40xlens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).

Flow Cyotometry of PBMC.  1X10^6 human PBMC were stained with anti-human CD86 antibody (65156-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG1 (KFA027) and Streptavidin-APC. The cells were co-stained with either mouse IgG2b isotype control or anti-human CD14 antibody (CL488-65246).  Cells are not fixed, Monocytes are gated.