Results

Cyclin D1 Monoclonal antibodies (2G3G5)

(Parent antibody plus 4 derived formats/conjugates)

KO/KD Validated

716 Publications

Reviews (3)

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat, bovine, goat, hamster, rabbit, zebrafish

Applications

ELISA, FC (Intra), IF/ICC, IP, WB

Conjugation

Cardinal Red™, CoraLite® Plus 488, CoraLite® Plus 647, Unconjugated

CloneNo.

2G3G5
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Unconjugated parent antibody
Cat No. 60186-1-Ig

Other conjugate/format antibodies
Cat No. CL647-60186, CR-60186, CL488-60186, 60186-1-PBS
Cyclin D1 Monoclonal antibodies (2G3G5) (5 total products)
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Cyclin D1 Polyclonal antibody

KO/KD Validated

404 Publications

View More Images (9)

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Various lysates were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, bovine, canine, goat, pig, sheep

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Sizes
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Cat No. 26939-1-AP
Cyclin D1 Polyclonal antibody
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Various lysates were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Cyclin D1 (26939-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (26939-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cyclin D1 antibody (26939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin D1 (26939-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Non-treated HeLa and thymidine treated HeLa cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.
Cyclin D1 Recombinant antibodies (6B10)

(Parent antibody plus 1 derived formats/conjugates)

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

ELISA, IF/ICC, IHC, WB

Conjugation

Unconjugated

CloneNo.

6B10
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Unconjugated parent antibody
Cat No. 82681-1-RR

Other conjugate/format antibodies
Cat No. 82681-1-PBS
Cyclin D1 Recombinant antibodies (6B10) (2 total products)
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Cyclin D1 Matched Antibody Pairs^New

Renewable, reliable, and scalable: rabbit recombinant and mouse monoclonal antibody production.
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Multiple pairs per target: find the best pair for your assay.

Pair Cat No.	Capture antibody	Detection antibody	Target	Species reactivity	Validated Applications	Linear Range
MP50463-1	68986-1-PBS	68986-2-PBS	Cyclin D1	Human	Cytometric bead array	CBA: 0.098-100 ng/mL
MP80003-1	68790-1-PBS	82681-1-PBS	Cyclin D1	Human	Sandwich ELISA	sELISA: 0.188-12 ng/mL

Human Cyclin D1 ELISA Kit

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Assay Type

Sandwich

Sample Type

Cell lysate

Sensitivity

0.16 ng/mL

Range

0.25-16 ng/mL

Sizes
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Cat No. KE00344
Human Cyclin D1 ELISA Kit
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More validation images available on the product page

Standard Curve of KE00344 Human Cyclin D1 ELISA Kit
Cyclin D1 E.Coli expressed Tagged-proteins

Species

human

Source

PET28a, PET30a, PGEX-4T

Tag

6*His, GST
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Cat No. Ag23241, Ag25384, Ag25393, Ag23228, Ag19109
Cyclin D1 E.Coli expressed Tagged-proteins (5 total products)
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CCNDBP1 Polyclonal antibody

11 Publications

View More Images (3)

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HeLa cells were subjected to SDS PAGE followed by western blot with 12363-1-AP (CCNDBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Sizes
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Cat No. 12363-1-AP
CCNDBP1 Polyclonal antibody
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×

The search results gallery shows a selection of our total image library
More validation images available on the product page

HeLa cells were subjected to SDS PAGE followed by western blot with 12363-1-AP (CCNDBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12363-1-AP (CCNDBP1 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12363-1-AP (CCNDBP1 Antibody) at dilution of 1:200 (under 40x lens).
IHCeasy CCNDBP1 Ready-To-Use IHC Kit

View More Images (6)

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Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using KHC2138 (CCNDBP1 IHC Kit).

Primary Ab Type

Rabbit Polyclonal

Reactivity

human, mouse, rat

Sample Type

FFPE tissue

Secondary Ab Type

Polymer-HRP-Goat anti-Rabbit

Sizes
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Cat No. KHC2138
IHCeasy CCNDBP1 Ready-To-Use IHC Kit
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×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using KHC2138 (CCNDBP1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC2138 (CCNDBP1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using KHC2138 (CCNDBP1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using KHC2138 (CCNDBP1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using KHC2138 (CCNDBP1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using KHC2138 (CCNDBP1 IHC Kit).
CCNDBP1 Fusion Protein

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Species

human

Source

E. coli-derived, T-HIS

Tag

6*His

Format

Powder

Sizes
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Cat No. Ag3032
CCNDBP1 Fusion Protein
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The search results gallery shows a selection of our total image library
More validation images available on the product page

CCNDBP1 fusion protein Ag3032 SDS-PAGE

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Cyclin D1 Monoclonal antibodies (2G3G5)

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Cyclin D1 Polyclonal antibody

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Cyclin D1 Recombinant antibodies (6B10)

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Cyclin D1 Matched Antibody Pairs^New

Human Cyclin D1 ELISA Kit

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Cyclin D1 E.Coli expressed Tagged-proteins

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CCNDBP1 Polyclonal antibody

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IHCeasy CCNDBP1 Ready-To-Use IHC Kit

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CCNDBP1 Fusion Protein

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Cyclin D1 Monoclonal antibodies (2G3G5)

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Cyclin D1 Polyclonal antibody

-_- / -_-

-_- / -_-

Cyclin D1 Recombinant antibodies (6B10)

-_- / -_-

-_- / -_-

Cyclin D1 Matched Antibody PairsNew

Human Cyclin D1 ELISA Kit

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Cyclin D1 E.Coli expressed Tagged-proteins

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CCNDBP1 Polyclonal antibody

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IHCeasy CCNDBP1 Ready-To-Use IHC Kit

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CCNDBP1 Fusion Protein

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Products derived from

Cyclin D1 Matched Antibody Pairs^New