Live cell imaging of HeLa cells: Live cell imaging of HeLa cells stable expressing Cell Cycle-Chromobody (TagRFP). Images from a time series of 22 hours are shown
Cell Cycle-Chromobody signal during the cell cycle: In G1, the Chromobody signal is homogeneously distributed throughout the nucleus and cytoplasm. During S phase it accumulates in the nucleus and visualizes the formation of replication foci. In G2, the foci disappear and the cell divides (mitosis).
Time lapse video: Live cell imaging of the stable cell line HeLa-Cell Cycle Chromobody TagRFP expressing Cell Cycle-Chromobody.
Nuclear Actin-Chromobody: NIH 3T3 cells (murine fibroblasts) transfected with the nuclear Actin-Chromobody (TagGFP2, green) and the Lamin-Chromobody (TagRFP, red) were starved in serum-free medium for 24 hours. Nuclear Actin-Chromobody probed endogenous F-actin assembly and distribution is shown 30 s after stimulation with serum. Courtesy of Matthias Plessner.
The time lapse analysis reveals the reorganization of actin after treatment with Cytochalasin D: HeLa cells were subjected to confocal imaging upon treatment with 2 µM of Cytochalasin D for 1 hour and recovery for 4 hours. Actin Chromobody enables monitoring of actin dynamics in real-time in living cells.
Time series of imaging DNA damage in HeLa cells after microirradiation: The PARP1-Chromobody allows real-time monitoring of PARP1 recruitment to the location of DNA damage after microirradiation (yellow triangle) in living cells.
Series of 4 images of HeLa cells expressing the Dnmt1-Chromobody (over 5.5 hours): In G phase cells, the signal is homogeneously distributed through the nucleus and cytoplasm. Over time, during S phase granules appear in the nucleus as replication foci form, finally the granularity disappears, and the cells divide.
Time series of imaging DNA damage in HeLa cells after microirradiation: The PARP1-Chromobody allows real-time monitoring of PARP1 recruitment to the location of DNA damage after microirradiation (yellow triangle) in living cells.
Series of 4 images of HeLa cells expressing the Dnmt1-Chromobody (over 5.5 hours): In G phase cells, the signal is homogeneously distributed through the nucleus and cytoplasm. Over time, during S phase granules appear in the nucleus as replication foci form, finally the granularity disappears, and the cells divide.
Live-cell imaging of Hela cells expressing Histone-Chromobody. Scale bar, 10 µm
Time-lapse imaging of HeLa cells expressing the Histone-Chromobody: HeLa cells expressing Histone-Chromobody throughout the cell cycle. Interphase chromatin, chromatin condensation and mitotic chromosomes are visible. Scale bar, 10 µm
Fluorescence microscopy of HeLa cells, transiently expressing Vimentin-mNeonGreen (green) and the mNeonGreen-Chromobody-(mCherry) (red). Nuclei are stained with DAPI (cyan).
Green and red fluorescent signals co-localize in co-transfected cells, indicating enrichment of the Chromobody at the mNeonGreen-positive structures. Confocal images were were acquired with a 63x oil objective and post-processed.
