1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human B2M Rabbit Recombinant Antibody (98165-1-RR, Clone:241308D5) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
Biolayer interferometry (BLl) kinetic assays of 98165-1-RR against Human Beta-2-Microglobulin were performed. The affinity constant is 0.743 nM.
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human B2M Rabbit Recombinant Antibody (98165-1-RR, Clone:241308D5) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98165-1-PBS in a different storage buffer formulation.
Biolayer interferometry (BLl) kinetic assays of 98165-1-RR against Human Beta-2-Microglobulin were performed. The affinity constant is 0.743 nM.
1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.5 ug PE Anti-Mouse IFN gamma (PE-65153, Clone: XMG1.2).
Immunofluorescent analysis of un-fixed HUVEC cells using CD146 antibody (65181-1-Ig, Clone: P1H12 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).
1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human CD146 (65181-1-Ig, Clone:P1H12) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000. Cells were not fixed.
1x10^6 unstimulated or PMA and ionomycin stimulated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug FITC Plus Anti-Mouse IFN-gamma (XMG1.2) (FITC-65153, Clone: XMG1.2) and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 untreated or LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) (red) or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
Biolayer interferometry (BLl) kinetic assays of 98087-1-RR
against Human IL-6 were performed. The affinity constant is 0.322 nM.
1x10^6 untreated or LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 98087-1-PBS in a different storage buffer formulation.
1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) (red) or Isotype Control (blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 98087-1-PBS in a different storage buffer formulation.
Biolayer interferometry (BLl) kinetic assays of 98087-1-RR
against Human IL-6 were performed. The affinity constant is 0.322 nM.
1x10^6 unstimulated or PMA and ionomycin stimulated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.5 ug Anti-Mouse IFN gamma (65153-1-Ig, Clone: XMG1.2) and FITC anti-rat IgG1 Antibody. Cells were co-stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR, Clone:240262C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Biolayer interferometry (BLl) kinetic assays of 98045-1-RR against Mouse IFN-gamma were performed. The affinity constant is below 1 pM.
1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR, Clone:240262C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98045-1-PBS in a different storage buffer formulation.
Biolayer interferometry (BLl) kinetic assays of 98045-1-RR against Mouse IFN-gamma were performed. The affinity constant is below 1 pM.
1X10^6 A375 cells were surface stained with 5 ul Atlantic Blue™ Anti-Human CD146 (AB-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul Atlantic Blue™ Anti-Human CD146 (AB-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul Cardinal Red™ Anti-Human CD146 (CR-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul Cardinal Red™ Anti-Human CD146 (CR-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD146 (CL405-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD146 (CL405-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 405 Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (CL405-98045, Clone:240262C9). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD146 (CL488-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD146 (CL488-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse IFN gamma (CL488-65153, Clone: XMG1.2).
1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (CL488-98045, Clone: 240262C9). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD146 (CL555-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD146 (CL555-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD146 (CL647-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD146 (CL647-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.
1x10^6 PMA and ionomycin Stimulate C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Mouse IFN gamma (CL647-65153, Clone:XMG1.2), and 0.13 ug CoraLite® Plus 647 Rat IgG1 Isotype Control (HRPN) (CL647-65212, Clone: HRPN), and 0.13 ug PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
