Various lysates were subjected to SDS PAGE followed by western blot with 25887-1-AP (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293T cells using Chk1 antibody (25887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

IP result of anti-Chk1 (IP:25887-1-AP, 4ug; Detection:25887-1-AP 1:600) with HEK-293T cells lysate 4000ug.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens).

1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Chk1 (25887-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28805-1-AP (Phospho-Chk1 (Ser296) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 28803-1-AP (Phospho-Chk1 (Ser345) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-Chk1 (Ser345) antibody (28803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Non-treated HEK-293T and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28807-1-AP (Phospho-Chk1 (Ser317) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1427 (CHK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC1427 (CHK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using KHC1427 (CHK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using KHC1427 (CHK1 IHC Kit).