K-562 cells were subjected to SDS PAGE followed by western blot with 10743-1-AP (Centromere protein R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP result of anti-Centromere protein R (IP:10743-1-AP, 4ug; Detection:10743-1-AP 1:300) with K-562 cells lysate 2400 ug.
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10743-1-AP (Centromere protein R antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Centromere protein R antibody (10743-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).