Various lysates were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB result of CD146/MCAM antibody (17564-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD146/MCAM transfected HepG2 cells.
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 40x lens).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug.
Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Anti-Human CD146 (P1H12) antibodies
(Parent antibody plus 9 derived formats/conjugates)
4 Publications
Host/IsoType
Mouse/IgG1, kappa
Reactivity
human, mouse, Canine, Rabbit
Applications
FC, IF/ICC
Conjugation
Atlantic Blue™, Cardinal Red™, CoraLite® Plus 405, CoraLite® Plus 488, CoraLite® Plus 555, CoraLite® Plus 647, CoraLite® Plus 750, FITC Plus, PE, Unconjugated