Various lysates were subjected to SDS PAGE followed by western blot with 18495-1-AP (CD133 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

HT-29 cells were subjected to SDS PAGE followed by western blot with 19946-1-AP (CD133-1,2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IP result of anti-CD133-1,2 (IP:19946-1-AP, 4ug; Detection:19946-1-AP 1:300) with Y79 cells lysate 2000ug.

Y79 cells were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

IP result of anti-CD133-1,2,4,7 (IP:19945-1-AP, 4ug; Detection:19945-1-AP 1:300) with Y79 cells lysate 2000ug.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).