Various lysates were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

HEK-293 (WT and ATXN3 KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 13505-1-AP incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 40x lens).

HEK-293 lysates prepared and IP of ATXN3 performed using 1.0 μg of 13505-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 40x lens).

Immunofluorescent analysis of Hela cells, using ATXN10 antibody 15693-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP result of anti-ATXN10 (IP:15693-1-AP, 4ug; Detection:15693-1-AP 1:1000) with A431 cells lysate 1200ug.

Ataxin 2 fusion protein Ag16470 SDS-PAGE

ATX3,ATXN3 fusion protein Ag6627 SDS-PAGE