HEK-293 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 28021-1-AP (ATG14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28021-1-AP (ATG14 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-ATG14/Barkor (IP:28021-1-AP, 4ug; Detection:28021-1-AP 1:500) with rat brain tissue lysate 4000 ug.
WB result of ATG14/Barkor (C-terminal) antibody (24412-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG14/Barkor (C-terminal) transfected HeLa cells.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human brain slide using 24412-1-AP (ATG14/Barkor (C-terminal) antibody at dilution of 1:50 (under 40x lens).
Immunofluorescent analysis of HeLa cells using 24412-1-AP (ATG14/Barkor (C-terminal) antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
