HeLa cells were subjected to SDS PAGE followed by western blot with 14834-1-AP (ATF2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14834-1-AP (ATF2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 11908-1-AP (ATF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-ATF2 (IP:11908-1-AP, 4ug; Detection:11908-1-AP 1:300) with mouse brain tissue lysate 3200 ug.

Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF2 antibody (11908-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11908-1-AP (ATF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Non-treated NIH/3T3 cells and Anisomycin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28790-1-AP (Phospho-ATF2 (Thr71)/ATF7 (Thr53) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Purity of recombinant human GDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
 

GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

Proteintech GDNF (HZ-1311) demonstrates a greater fold induction of proliferation compared to a leading competitor at an equivalent EC50. GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta
 Johannes Kepler Universitat)

Various lysates were subjected to SDS PAGE followed by western blot with 26179-1-AP (GDNF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26179-1-AP (GDNF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26179-1-AP (GDNF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GDNF antibody (26179-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

GDNF fusion protein Ag24192 SDS-PAGE