Various lysates were subjected to SDS PAGE followed by western blot with 28201-1-AP (ASK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28201-1-AP (ASK1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ASK1 Monoclonal antibodies (1C3A10)
(Parent antibody plus 2 derived formats/conjugates)
Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28846-1-AP (Phospho-ASK1 (Ser966) antibody) at dilution of 1:500 incubated at 4℃ overnight.
Phosphatase inhibitor treated and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28847-1-AP (Phospho-ASK1 (Ser83) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.